...of the manager is not to empower but to remove obstacles.” – Scott Adams, Dilbert cartoonist What makes...for example. So aside from a focus on removing obstacles, what makes a good manager? First, it takes an...their present positions By removing the primary obstacle of job dissatisfaction, the work can get done,...

...conversation about GMOs.  Challenge #2: Technical obstacles to plant synthetic biology In addition to public...understanding of plant biology is a significant technical obstacle to plant synthetic biology. We cannot build what...color-changing flower, and we’d love to hear about the obstacles you’ve faced as a plant scientist, and how you...

...code of life. In 1944, Oswald Avery, Colin MacLeod, and Maclyn McCarty’s experiment debunked the myth that...://doi.org/10.1038/s41586-019-1711-4 Avery OT, MacLeod CM, McCarty M (1944) STUDIES ON THE CHEMICAL NATURE...

...as though nothing is a miracle. The other is as though everything is a miracle.” This quote encourages...

... function in high salt conditions - up to 100 mM NaCl. These tTALE-FPs also have higher DNA binding affinity...regions vs. GC-rich regions from 40 mM to 100 mM NaCl but stained evenly in 1x TE buffer. The lab also...

...nothing if not solving your way through a series of obstacles in pursuit of achieving knowledge. Or, as we in...result of competing desires or (3) the presence of obstacles that need to be overcome. Conflict, and its inherent...

...diseases, blindness, and diabetes. Directions and obstacles for future CRISPR therapies CRISPR technology ...systemic approaches, has its own unique set of obstacles. Thus far, several targeted therapies have taken...

...how CRISPR can take on HIV-1 in vitro, and what obstacles must be overcome for clinical success. ART and...ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR HIV-1 therapy Both of these methods...

...for at least six months. Future directions and obstacles to clinical CRISPR editing Given the success of...diseases to be treated clinically with CRISPR. Many obstacles remain for CRISPR gene editing, but given the ...

...with any integrated transgene). To overcome this obstacle, a positive selection cassette can be incorporated...use of negative selection largely overcomes this obstacle. Furthermore, we have found that transfecting ...

... into mammalian cells often relied on chemical (CaCl2, cationic liposome) or electrical (electric current... you don’t have any experience in transfection, CaCl2 transfection is a simple and cheap technique that...

...synthetic biology, synthetic chromosomes, and obstacles to becoming an academic researcher. Listen to...

...with lab members, determine how to work through obstacles, and take steps to resolve interpersonal conflicts...

...improve CTC capture, but I hope that our antibody tentacles may have an impact on this challenge either for...

...of our inner children who have no preconceived obstacles, optimistically grasp at limitless potential, ...

...the work gets done Managers do this by removing obstacles that stand in the way of the doers and by ensuring...

... ours reflect a growing effort to address this obstacle in its many forms. A short stroll from my office...

...protein enter cells. Subsequent work found that both NaCl and non-detergent sulfobetaine 201 (NDSB-201) were...

...supernatant is collected, filtered, and titered. A major obstacle in obtaining usable lentivirus is producing a ...

...that have helped me troubleshoot some technical obstacles when working with virus: 1. Viral vectors undergo...