...Pluronic-F68 NaCl MgCl 2 KCl Centrifugal filter units (MWCO 100 kDa) Reagent Preparation 1 M NaCl/PBS-MK buffer...in PBS + 200 mM NaCl (C) (formulation buffer) Add 5 mL of Buffer B and 2 mL of 5 M NaCl to 43 mL PBS Procedure...impure AAV preparation. The 15% iodixanol step has 1M NaCl to destabilize ionic interactions between macromolecules... buffer Dissolve 5.84 g of NaCl, 26.3 mg of MgCl 2 and 14.91 mg of KCl in 1× PBS in a final volume of ...: mix 4.5 mL of 60% iodixanol and 13.5 mL of 1 M NaCl/PBS-MK buffer 25% iodixanol step: mix 5 mL of 60%... A C: 0.001% in PBS: 45 mL PBS + 5 ml B + 200 mM NaCl Procedure Cover the filter membrane with 15 mL of...through. Add 15 mL of 0.001% Pluronic F68 + 200mM NaCl PBS. Spin at 3000 rpm for 5 min at 4 °C and discard...

...Glycol 8000 (PEG) + 0.5 M NaCl Cell lysis buffer (50 mM Tris HCl, 150 mM NaCl, 2 mM MgCl2) Reagent Preparation...solution: Dissolve 400 g of PEG 8000 and 24 g of NaCl into deionized water and adjust to a final volume... °C. Cell Lysis Buffer : 50 mM Tris HCl, 150 mM NaCl, 2 mM MgCl 2 Add the following to the 2 L sterile...

... in annealing buffer (10mM Tris, pH7.5-8.0, 50mM NaCL, 1mM EDTA) and mixed in equimolar concentrations...

...out the following into a 500 mL glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH 2 O to 400...

...polybrene (Sigma-Aldrich catalog #H9268) in 0.9% NaCl. Autoclave to sterilize. Stock solution is stable...