...applied to APC-Cy7 against APC to correct for this bleed. A compensation value of 3.7 for APC into APC-Cy7 applied...you will need compensation between APC and APC-Cy7; PE and PE-Cy7; APC-Cy7 and PE-Cy7; or BV421 and BV51...voltages.  Figure 1A shows a single-color APC control, with APC and APC-CY7 plotted against each other. Since... out of APC-Cy7. After increasing the compensation values for APC against APC-Cy7, the populations are...only be positive for APC, yet it seems as if the beads are also positive for APC-Cy7. You therefore would...would say that APC is “bleeding,” or spilling over, into APC-Cy7 channel and needs to be compensated out... showing an APC single-color control sample before (A) and after (B) compensation with APC-Cy7. The positive...

...Figure 3), we see that APC-Cy7 is excited at a lower efficiency than APC. While APC's global excitation maximum...conjugated single fluorophores, like APC-Cy7. Conjugating Cy7 to APC results in a higher-wavelength emission... that excites at 750 nm, APC-Cy7 would show a much brighter signal, while APC would show no signal at ...Figure 3: Excitation and emission spectra for APC and APC-Cy7. While the excitation spectra of both fluorophores...through the actual APC-Cy7 fluorophore but displays an emission spectrum that overlaps with APC-Cy7. Therefore...through the APC-Cy7 parameter in the cytometer's software. It also means we should exclude APC-Cy7 from ...emission spectrum compared to APC. This happens due to a process called Fluorescence Resonance Energy Transfer...

...single color control for the APC channel. B) and C) A flow plot showing an APC single color control prior...staining for anti-mouse Ly6C in APC with the IgG2c subclass, you will use the APC IgG2c isotype control). The...compensation (B) and after compensation (C) with APC-Cy7.   FMOs FMO stands for fluorescence minus...

...: Suppressing Function Antigen presenting cells (APCs) that would normally alert the rest of the immune...growth factor beta (TGFβ) and IL-10 that suppress APCs. T cells that would normally attack the cancer cells... turns off T cell function upon interaction with APCs. Furthermore, tumor cells alter their cell-surface...proximity to the tumor; chemokine-aided activation of APCs produces modified dendritic cells (DCs) and macrophages...

...Association for Congenital Disorders of Glycosylation (APCDG) and an international platform for CDG research,...