...specific ncRNA loci to enhance or upregulate ncRNA expression. This approach is useful for lncRNAs because...target ncRNA. CRISPR/Cas9 techniques and targeting methods Knock-out: Targeting short vs. long ncRNAs The...trickier when it comes to long non-coding RNAs (lncRNA). lncRNAs are over 200 nucleotides in length and lack...thousands of long ncRNAs at the same time (Liu et al. 2018). However, removing large lncRNA fragments with... visualizing ncRNA localization and function, but directly incorporating very long ncRNAs is impractical...that can target ncRNAs.  CRISPRi and CRISPRa For scientists who want to modulate ncRNA expression without...study ncRNA, CRISPR systems offer a number of different options. By carefully considering your ncRNA of ...

...cell technologies, such as single cell RNA-seq (scRNA-seq), now allows scientists to identify the gene...cells that successfully reprogram. Unfortunately scRNA-seq only captures the gene expression of cells at...identifiers act like a timestamp and help demultiplex the scRNA-seq data and build multi-generational cell lineage...three days, a sample of cells was collected for scRNA-seq analysis and the remaining cells were maintained... a combinatory cell-indexing tool that combines scRNA-seq and lineage tracking to track the dynamics of...

...domains. Combined with dCas9, this new scaffold RNA (scRNA) will bind to a PAM-adjacent target nucleotide sequence...simultaneous and multidirectional multiplex control via scRNA design is readily apparent in metabolic engineering...violacein. Using dCas9 as an on/off switch The multiple scRNAs all individually utilize the same dCas9 protein...promoter to modulate dCas9 expression, multiple scRNA transcriptional programs can be regulated with dCas9...

...defect. They have created a circRNA website so scientists can view public circRNA datasets and download the... discover circRNAs in RNA-seq data. As far as I can tell, we don’t really know what circRNAs do yet or...

...RNAs (lncRNAs) in human health and disease. A primary question in their quest to understand lncRNAs: What...What can lncRNAs do by themselves? The Rinn lab has developed a novel technique, called CRISPR-Display,...aptamers, pools of random RNA sequences, and natural lncRNAs to specific locations in the genome. Furthermore...

...replication. ColIb-P9 copy number is regulated by an lncRNA. RepZ recruits host proteins to the ori to initiate...pseudoknot. At high copy numbers, high levels of the lncRNA bind to RepZ mRNA and prevent this pseudoknot from...

... ~3 kb ~3.9kb crRNA/gRNA length gRNA: ~100 nt crNA: ~42 nt Requires tracrRNA? Yes No dsDNA cleavage...

...genome has been full of dark matter: enhancers, lncRNAs, repetitive elements, repressors, insulators, and...

...through isothermal amplification by RPA. When Cas12a-cRNA base pairs with the dsDNA of interest, the DNase...

...the presence of two or more mismatches in the cRNA:target duplex and will not give a positive result for...

...Neurobiology, 4, 100086. https://doi.org/10.1016/j.crneur.2023.100086. Challis, R. C., Ravindra Kumar, ...