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  1. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...specific ncRNA loci to enhance or upregulate ncRNA expression. This approach is useful for lncRNAs because...target ncRNA. CRISPR/Cas9 techniques and targeting methods Knock-out: Targeting short vs. long ncRNAs The...trickier when it comes to long non-coding RNAs (lncRNA). lncRNAs are over 200 nucleotides in length and lack...thousands of long ncRNAs at the same time (Liu et al. 2018). However, removing large lncRNA fragments with... visualizing ncRNA localization and function, but directly incorporating very long ncRNAs is impractical...that can target ncRNAs.  CRISPRi and CRISPRa For scientists who want to modulate ncRNA expression without...study ncRNA, CRISPR systems offer a number of different options. By carefully considering your ncRNA of ...

  2. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...cell technologies, such as single cell RNA-seq (scRNA-seq), now allows scientists to identify the gene...cells that successfully reprogram. Unfortunately scRNA-seq only captures the gene expression of cells at...identifiers act like a timestamp and help demultiplex the scRNA-seq data and build multi-generational cell lineage...three days, a sample of cells was collected for scRNA-seq analysis and the remaining cells were maintained... a combinatory cell-indexing tool that combines scRNA-seq and lineage tracking to track the dynamics of...

  3. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...domains. Combined with dCas9, this new scaffold RNA (scRNA) will bind to a PAM-adjacent target nucleotide sequence...simultaneous and multidirectional multiplex control via scRNA design is readily apparent in metabolic engineering...violacein. Using dCas9 as an on/off switch The multiple scRNAs all individually utilize the same dCas9 protein...promoter to modulate dCas9 expression, multiple scRNA transcriptional programs can be regulated with dCas9...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...RNAs (lncRNAs) in human health and disease. A primary question in their quest to understand lncRNAs: What...What can lncRNAs do by themselves? The Rinn lab has developed a novel technique, called CRISPR-Display,...aptamers, pools of random RNA sequences, and natural lncRNAs to specific locations in the genome. Furthermore...

  6. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...replication. ColIb-P9 copy number is regulated by an lncRNA. RepZ recruits host proteins to the ori to initiate...pseudoknot. At high copy numbers, high levels of the lncRNA bind to RepZ mRNA and prevent this pseudoknot from...

  12. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Human Emerman OhAinle 3rd 8 15,416 Human CRISPR lncRNA Activation Pooled Library 1000000106 Activation...191535 Knockout Human Olzmann 3rd ~10 13,920 Human lncRNA Splicing-targeting CRISPR Library 119977 Knockout... RNA (pgRNA) Library for Long Non-coding RNAs (lncRNAs) 89640 Knockout Human Wei 3rd Varies 12,472 pairs...Stiewe N/A Varies 1,910–3,034 per exon Human Cas13 lncRNA Library 227014 RNA Knockdown Human Sanjana 3rd ...Knockout Mouse Pu N/A 6 14,671 Pan-cancer CasRx lncRNA Albarossa Library 212966 Knockout Human Rad 3rd...

  14. Retrovirus Plasmids

    Type
    Collection
    ...plasmids in this toolkit. Lowe 64865 pLncEXP CMV/MSV lncRNA expression plasmid Sun 60683 pLXIN-Luc MoMSV Stable...

  15. Brain Armamentarium

    Type
    Collection
    ...labeling in neurons. Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting Viviana Gradinaru...

  16. Luciferase Plasmid Collection

    Type
    Collection
    ...For use as a reporter of the ability of a given lncRNA to cis-repress Mauro Calabrese 106253 pLS-mP-Luc...

  17. Brain Initiative Collection

    Type
    Collection
    ...labeling in neurons. Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting 163909-AAV9 pAAV_hSynapsin_psychLight2...

  18. CRISPR Guide

    Type
    Collection
    ...arrays of multiple gRNAs to target paralogs or lncRNAs . Guide RNAs are designed in silico and synthesized...

  19. CRISPR Guide

    Type
    Guide
    ...arrays of multiple gRNAs to target paralogs or lncRNAs . Guide RNAs are designed in silico and synthesized...
Showing: 1 - 20 of 20 results