... to a fluorochrome. In indirect staining a primary antibody binds to a target and a fluorochrome-conjugated...associated fluorophore or stained with the associated fluorochrome, emits a fluorescent signal that is captured...expressing high levels of the fluorophore or fluorochrome-stained protein will emit a stronger photon ...using this approach, avoid fixation-sensitive fluorochromes such as phycoerythrin (PE) and allophycocyanin...same Ig subclass, and conjugated to the same fluorochrome as your primary antibody. The isotype control...correcting spectral overlap that occurs between fluorochromes. For example, FITC dye emits green, yellow and...compensation the signal arising from a specific fluorochrome is removed from all other detectors except its...