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  1. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ... to a fluorochrome. In indirect staining a primary antibody binds to a target and a fluorochrome-conjugated...associated fluorophore or stained with the associated fluorochrome, emits a fluorescent signal that is captured...expressing high levels of the fluorophore or fluorochrome-stained protein will emit a stronger photon ...using this approach, avoid fixation-sensitive fluorochromes such as phycoerythrin (PE) and allophycocyanin...same Ig subclass, and conjugated to the same fluorochrome as your primary antibody. The isotype control...correcting spectral overlap that occurs between fluorochromes. For example, FITC dye emits green, yellow and...compensation the signal arising from a specific fluorochrome is removed from all other detectors except its...
  2. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...Matlashov et al., 2020) derived from bacterial phytochrome (BphPs) photoreceptors have higher apparent cellular...FPs, named miRFPnanos, were derived from cyanobacteriochrome (CBCR) photoreceptors (Oliinyk et al., 2019...fluorescent proteins engineered from bacterial phytochromes. Curr Opin Chem Biol 27, 52-63 (2015). Shcherbakova...near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing...
  3. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...Resources References Boston University Flow Core. Fluorochromes Brightness Chart. Boston University Flow Core...https://www.bu.edu/flow-cytometry/files/2013/06/Fluorochromes-Brightness-Chart.pdf Syracuse Flow Core. Syracuse...
  4. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...reports that described magnetic responses of cryptochrome proteins or organic fluorophores (Karki et al...N., Vergish, S., & Zoltowski, B. D. (2021). Cryptochromes: Photochemical and structural insight into magnetoreception...
  5. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...oxygen, and voltage (LOV) domains, phytochrome B (PhyB), and cryptochrome 2 (CRY2) (Zhang & Cui, 2015). When...
  6. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...based on the light-inducible heterodimerizing cryptochrome 2 (CRY2) and calcium and integrin-binding protein... N-Cas9, Haifeng Ye’s lab used the bacterial phytochrome BphS which produces c-di-GMP in response to far-red...
  7. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...: "First, the light-responsive molecules of cryptochrome 2 are the chromophores flavin-adenine dinucleotide...
Showing: 1 - 20 of 21 results