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  1. Optogenetics AAV Preps

    Type
    Collection
    ...dependent 9 Harvey 108912 pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 CAG ChroME (soma-targeted) mRuby3 Cre dependent... ChR2/other mutants Chronos, Chrimson, ChRmine, ChroME, ChETA, C1V1 (t/t) Inhibitory Arch and variants...
  2. Brain Initiative Collection

    Type
    Collection
    ...108912-AAV9 pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 Cation channelrhodopsin ChroME targeted to the neuronal...
  3. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ... to a fluorochrome. In indirect staining a primary antibody binds to a target and a fluorochrome-conjugated...associated fluorophore or stained with the associated fluorochrome, emits a fluorescent signal that is captured...expressing high levels of the fluorophore or fluorochrome-stained protein will emit a stronger photon ...using this approach, avoid fixation-sensitive fluorochromes such as phycoerythrin (PE) and allophycocyanin...same Ig subclass, and conjugated to the same fluorochrome as your primary antibody. The isotype control...correcting spectral overlap that occurs between fluorochromes. For example, FITC dye emits green, yellow and...compensation the signal arising from a specific fluorochrome is removed from all other detectors except its...
  4. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...Matlashov et al., 2020) derived from bacterial phytochrome (BphPs) photoreceptors have higher apparent cellular...FPs, named miRFPnanos, were derived from cyanobacteriochrome (CBCR) photoreceptors (Oliinyk et al., 2019...fluorescent proteins engineered from bacterial phytochromes. Curr Opin Chem Biol 27, 52-63 (2015). Shcherbakova...near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing...
  5. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...Resources References Boston University Flow Core. Fluorochromes Brightness Chart. Boston University Flow Core...https://www.bu.edu/flow-cytometry/files/2013/06/Fluorochromes-Brightness-Chart.pdf Syracuse Flow Core. Syracuse...
  6. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...reports that described magnetic responses of cryptochrome proteins or organic fluorophores (Karki et al...N., Vergish, S., & Zoltowski, B. D. (2021). Cryptochromes: Photochemical and structural insight into magnetoreception...
  7. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...based on the light-inducible heterodimerizing cryptochrome 2 (CRY2) and calcium and integrin-binding protein... N-Cas9, Haifeng Ye’s lab used the bacterial phytochrome BphS which produces c-di-GMP in response to far-red...
  8. Optogenetics Plasmid Collection

    Type
    Collection
    ...utilizing a light sensitive protein from cryptochromes, phytochromes, or light-oxygen-voltage-sensing (LOV...
  9. Let There Be LITE Plasmids

    Type
    Blog Post
    ...: "First, the light-responsive molecules of cryptochrome 2 are the chromophores flavin-adenine dinucleotide...
Showing: 1 - 20 of 27 results