Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 8 results for: EcoRI

Showing: 1 - 8 of 8 results

  1. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...graphically in the following figure, in which we add EcoRI and NotI sites to Your Gene of Interest (YGOI) for...will save time later In our example, we will use EcoRI and NotI to ligate our cDNA into the recipient plasmid...the region that binds the ORF and we will add the EcoRI restriction site (GAATTC) to the 5’ end of this ...

  2. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ... insert with EcoRI and HindIII and, when you mix the cut products together, the two EcoRI digested ends... (blue arrow) followed by the restriction sites EcoRI, XhoI, and HindIII. To place your gene in the proper...orientation downstream of the promoter, you can add an EcoRI site just 5’ of the start of the gene and a HindIII...

  3. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ...designed a PAM-out nickase experiment to insert an EcoRI site using a single-stranded oligonucleotide (ssODN...ssODN) donor. Using a D10A nickase to insert an EcoRI site in various spots between the nick sites, they...

  4. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...concocted a wild idea of using the newly discovered EcoRI enzyme (and its predictable cut site) to develop...developed kanamycin resistant plasmid, pSC102, with EcoRI and selected for E. coli transformants that were...

  5. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ... BioBricks SpeI and XbaI EcoRI and PstI Bglbricks BglII and BamHI EcoRI Additional Resources ...
Showing: 1 - 8 of 8 results