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We narrowed to 14 results for: FRT

Showing: 1 - 14 of 14 results

  1. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...recognizes FRT sites and initiates site directed gene recombination between those sites. FRT sites and ...the Cre-lox or one of the FLP-FRT systems in your experiments. Mutated FRT and loxP sites in Flex Vectors... by the coordinated use of fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally... been developed. Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and Cre enable...additional levels of genetic control. What is FLP FRT and how does it work? FLP recombinase, which is named...For details see Cre-lox blog post). Optimizing FLP FRT through smart engineering Different FLP versions ...Whereas the excision of a piece of DNA flanked by loxP/FRT sites is favored over its reintroduction, inversion...

  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...recognition target (FRT) site being analogous to Cre and loxP, respectively. The FLP-FRT technology can be... single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another site-directed recombination...

  3. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...Cre recombinase binds loxP sites, while FLP binds FRT sites. When a DNA sequence is flanked by target sites...you could use a pair of loxP sites and a pair of FRT sites and include both Cre and FLP recombinases. ...

  4. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... vector designs used a lox-STOP-lox (LSL) or frt-STOP-frt (FSF) sequence prior to an ATG start codon to...of specific sequences, lox (recognized by Cre) or frt (recognized by Flp), to either flip or excise a gene...

  10. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...the CNS and PNS Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Control Gene Expression...

  11. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...GAL4 architecture or combined the system with Flp/FRT, as reviewed by del Valle Rodriguez et al.   LexA...

  12. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...for those involving recombination elements such as FRT or Lox sites, and provides a modular system for easier...new versions. Flipbow employs the Flp recombinase/FRT system in place of Brainbow’s Cre/loxP, allowing ...
Showing: 1 - 14 of 14 results