Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 21 results for: FRT

Showing: 1 - 20 of 21 results

  1. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...recognizes FRT sites and initiates site directed gene recombination between those sites. FRT sites and ...the Cre-lox or one of the FLP-FRT systems in your experiments. Mutated FRT and loxP sites in Flex Vectors... by the coordinated use of fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally... been developed. Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and Cre enable...additional levels of genetic control. What is FLP FRT and how does it work? FLP recombinase, which is named...For details see Cre-lox blog post). Optimizing FLP FRT through smart engineering Different FLP versions ...Whereas the excision of a piece of DNA flanked by loxP/FRT sites is favored over its reintroduction, inversion...

  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...recognition target (FRT) site being analogous to Cre and loxP, respectively. The FLP-FRT technology can be... single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another site-directed recombination...

  3. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...Cre recombinase binds loxP sites, while FLP binds FRT sites. When a DNA sequence is flanked by target sites...you could use a pair of loxP sites and a pair of FRT sites and include both Cre and FLP recombinases. ...

  4. Deisseroth INTRSECT Collection

    Type
    Collection
    ...recombinase recognition sequences (e.g. Lox sites, FRT sites) inside of the introns (A,D). The addition ...137162 pAAV-Ef1a-Con/Foff 2.0-EYFP Cre AND NOT Flp FRT/F5 Yes 55652 pAAV-hSyn Coff/Fon EYFP Flp AND NOT ...137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT Flp FRT/F5 Yes 137131 pAAV-Ef1a-Coff/Fon-BFP Flp AND NOT ...137133 pAAV-Ef1a-Con/Foff 2.0-mCherry Cre AND NOT Flp FRT/F5 Yes 137134 pAAV-Ef1a-Coff/Fon-mCherry Flp AND ... pAAV-Ef1a-Con/Foff 2.0-oScarlet Cre AND NOT Flp FRT/F5 Yes 137138 pAAV-Ef1a-Coff/Fon-oScarlet Flp AND...137120 pAAV-Ef1a-Con/Foff 2.0-GCaMP6M Cre AND NOT Flp FRT/F5 Yes 137121 pAAV-Ef1a-Coff/Fon-GCaMP6M Flp AND ...137123 pAAV-Ef1a-Con/Foff 2.0-GCaMP6F Cre AND NOT Flp FRT/F5 Yes 137124 pAAV-Ef1a-Coff/Fon-GCaMP6F Flp AND ...

  6. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... another FRT site. Flp recombinase recognizes and mediates recombination between the two FRT sites, resulting...+ 8 bp spacer (48 bp or 34 bp) F3, F5, FRT14, FRT15 minimal FRT GAAGTTCCTATTCtctagaaaGTATAGGAACTTC Table... a specific target site (called loxP for Cre, or FRT for Flp). The nature of the DNA rearrangement after...sites (for example, Cre with lox sites, Flp with FRT sites, Dre with rox sites), enabling precise genetic... only the canonical rox site is widely used. Flp FRT GAAGTTCCTATTCCGAAGTTCCTATTCtctagaaaGTATAGGAACTTC ...Addgene's blog on FLEx Vectors . Flp-IN: A single FRT site within the genome of a host cell line serves... gene of interest into the genome at the defined FRT site. Multiplexing: Orthogonal recombinase systems...

  12. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...the CNS and PNS Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Control Gene Expression...

  13. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...recombinases to remove genes flanked by either loxP or FRT sites respectively, and empty vectors to express ...

  15. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...for those involving recombination elements such as FRT or Lox sites, and provides a modular system for easier...new versions. Flipbow employs the Flp recombinase/FRT system in place of Brainbow’s Cre/loxP, allowing ...

  17. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Tabin 19457 pcDNA5/FRT/TO HSPA1L HSPA1L CMV Parkinson's Harm Kampinga 19484 pcDNA5/FRT/TO GFP HSPA1L HSPA1L... 19511 pcDNA5/FRT/TO V5 HSPA1L HSPA1L V5 CMV Parkinson's Harm Kampinga 19538 pcDNA5/FRT/TO HIS HSPA1L ...114193 pc DNA FRT TO- APP695 APP CMV Alzheimer's Aleksandra Radenovic 114194 pc DNA FRT TO- APPSwed/Ind...231174 pcDNA5 FRT TO GFP11-LRRK2 LRRK2 GFP CMV Parkinson's Samara Reck-Peterson 231175 pcDNA5 FRT TO GFP11-... 231583 pcDNA5/FRT-hMAPT MAPT CMV, T7 Parkinson's, FTD Chaolin Zhang 231584 pcDNA5/FRT-mMAPT MAPT CMV,...Norden 106093 pFRT_TO_FlagHA_TIA1 TIA1 Flag, HA CMV ALS Thomas Tuschl 106094 pFRT_TO_eGFP_TIA1 TIA1 GFP CMV...SNCA GFP TRE Parkinson's Vikram Khurana 209083 pcDNA_FRT_SNCA-Avi_HygR-2A-BirA.dna(TO_deleted). (pEHA1448...

  19. Adenovirus Guide

    Type
    Guide
    ...been developed, such as using the Cre-lox and Flp-FRT recombination systems to excise the packaging signal...
Showing: 1 - 20 of 21 results