... bind to GAL4, partially inhibiting the binding of GAL4 to UAS. One can therefore place GAL4 and GAL80...common binary system is the GAL4/UAS system isolated from yeast. In this system, UAS basal promoter expression...expression is low but is activated by GAL4 binding to UAS. If you place the GAL4 gene downstream of a tissue- ...to intermediate expression from QUAS, as seen with GAL4/GAL80 and UAS. QF-mediated repression is reversible...phase of yeast culture. Repressible Binary Systems GAL4/UAS In Drosophila or development studies, you may ... Lee. LexA/lexAop is a complementary system to GAL4/UAS that functions in essentially the same manner,...activators of lexAop. This system is commonly used with GAL4/UAS to examine the expression of reporter genes, or...

...1):  GAL4-UAS, a transcription factor binding site. GAL4 expressed in the fly binds to the UAS binding...SPARC Figure 1: SPARC and SPARC2 use the GAL4-UAS system along with precisely truncated attP sites...transcription activation systems, such as LexA/p65 or mCD8/GFP, to allow sparse labeling in a variety of fly cell...

...GBPs/GFP. The new system, CRE-DOG (Cre Dependent On GFP), is activated by GFP and derivatives GFP and ...to bind GFP. The idea of using GFP as a scaffold suddenly seemed very realistic. Envisioning GFP as a substrate...presence of GFP. These systems, known as T-DDOG and Cre-DOG, respectively, repurpose popular GFP reporter...lines expressing GFP in specific cell types to do more than just label cells. If GFP could be co-opted...selectively manipulate only GFP-labeled cells. Once Tang and Cepko found a description of GFP-binding nanobodies...tested pairs of their GFP-binding proteins (GBPs) to find those that could co-occupy GFP. Once they’d found...GBPa-VP16 (activation domain); GBPb-GAL4 (DNA-binding domain); and a UAS-driven luciferase reporter construct...

...transactivator is used. UAS General expression mRNA Yeast promoter containing Gal4 binding sites, commonly... in Drosophila Specific Requires the presence of Gal4 gene to activate promoter. Ac5 General expression... be used independently or together. Regulated by GAL4 and GAL 80. TEF1 General expression mRNA Yeast...Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org Find Plasmids for Your...

...integration into the fly genome and are under the Gal4 inducible UAS promoter. Other vectors can be expressed ...using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. pPD80_08) and following the expression pattern... allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating transiently...

...activation sequence (UAS) of its target gene, in this case a reporter gene (e.g. luciferase or GFP), regardless...Although the original Y2H systems utilized the yeast Gal4 activator, bacterial LexA DBD and the lacZ reporter...the binding sites for the protein partner or the UAS/reporter gene, the same bait and prey libraries can...