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We narrowed to 15 results for: GPCR

Showing: 1 - 15 of 15 results

  1. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...five senses? GPCRs or G-protein-coupled receptors (GPCRs)! Aside from these three senses, GPCRs play roles...signal through more than one subtype. How GPCR signaling works GPCR signaling is initiated when a ligand binds...extracellular surface of the GPCR. This results in a conformational change in the GPCR causing the activation... Addgene Tools for Studying GPCR signaling  So with the hundreds of GPCRs out there, how can scientists...activate many GPCRs are still unknown. To identify some of these unknown ligands and their GPCR binding partner...120 known GPCR targets demonstrating its potential to discover new ligands for unknown GPCRs. You can ...our blog post on GPCR signaling using TRUPATH for more information on this system! GPCR-APEX: Interested...

  2. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...intracellular surface of the GPCR. Binding of a ligand to the extracellular surface of the GPCR causes a conformational...understudied GPCRs in response to different agonists. These studies showed the promiscuity of GPCRs for different...they do so is through G protein-coupled receptors (GPCRs), receptors that play many roles in signaling processes...approximately 950 different human genes encoding GPCRs (Takeda S et al., 2002) and many downstream complexes...can be difficult for scientists to understand how GPCR activation leads to downstream effects.  Bryan ...transducerome) that can be activated downstream of GPCRs that are not involved in sight (Olsen RHJ et al....protein. This complex converts a signal received by a GPCR on the cell’s surface into action that can result...

  3. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...controlling GPCR activation have been developed to selectively study individual types of GPCRs with greater...be used for lower frequency stimulation. GPCR signaling GPCR signaling is a complicated process involving...change the membrane potential. OptoXRs are modified GPCRs. When exposed to light, they activate downstream... coupled receptors  G-protein coupled receptors (GPCR) regulate cellular function through multiple intra-cellular...have been developed for utilizing light to control GPCR activation and downstream signaling cascades (reviewed...Levitz, 2020), including light-sensitive chimeric GPCRS (OptoXRs) (Tichy et al., 2019), photoswitchable ...rhodopsin, and the intracellular domain of an endogenous GPCR, such as adrenergic receptors. When exposed to light...

  4. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...biosensors kit By Mike Lacy G protein-coupled receptors (GPCRs) are critical components of many physiological pathways...targets. To enable large-scale profiling of many GPCRs, Mikel Garcia-Marcos’ lab recently developed a collection... detection of activation triggered by endogenous GPCR. Example applications include large-scale pharmacological...The authors used this platform to assay dozens of GPCRs across many cell types in varying conditions, revealing...2024). Direct interrogation of context-dependent GPCR activity with a universal biosensor platform. Cell...

  5. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...signaling pathways G protein-coupled receptors (GPCRs) detect molecules outside of the cell and translate...those signals into cellular responses. For example, GPCRs and their downstream G protein partners can regulate...have a long standing interest in the biology of GPCRs and G proteins, with an emphasis on oligomerization...

  6. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...designer drugs, are G protein-coupled receptors (GPCRs), that have been reprogrammed to respond only to... threshold and silence neurons. DREADD-activated GPCR signaling can also modify other properties such ...

  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...target G-protein coupled receptors (GPCRs) yet one third of the GPCRs in the human genome are orphan receptors...are unknown. Identifying new ligands for orphan GPCRs is of significant medical interest, however efforts...allows researchers to interrogate the druggable human GPCR-ome using a quick, affordable, and ubiquitous reporter...recruitment assay incorporating 314 codon-optimized GPCR sequences for human cell line expression. In a twist...identified new, highly specific agonists for orphan GPCRs demonstrating the utility of this platform for drug...easily shuttle the optimized, expression validated GPCR sequences to any desired backbone.  Kroeze, et ...

  8. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ... loop regions of any G-protein-coupled receptor (GPCR) recognizing a specific ligand (Ino et al. 2022)...produce more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters, neuropeptides...

  11. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...Rhodopsin GEVIs Rhodopsin is composed of an opsin (a GPCR) linked to a chromophore and functions as a photoreceptor...

  12. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... of the new viral preps from recent months: New GPCR activation-based norepinephrine (GRABNE) sensors...

  15. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...new optogenetic tool called PdCO.  This is an "optoGPCR" (coupled to Go, so it's an inhibitory opsin!)...
Showing: 1 - 15 of 15 results