...western blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated...being purified by GST (maybe try MBP if you are having solubility issues!). Need a GST antibody? Addgene...sites immediately following the tag. Unlike MBP, the GST tag can be cleaved off from the fusion protein while...a handy feature. If you decide not to cleave the GST tag after/during purification, be aware that the ... affect downstream applications. Also, purifying GST by affinity chromatography requires proper folding...

...binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on a reduced...immobilized target protein. The disadvantage is that the GST moiety, which forms dimers, may influence binding...experimental protein-ligand interaction approaches that of GST-glutathione. Biotin-based immobilization for kinetic...avidin can be controlled easier than formation of GST dimers. Avi-Tag plasmid for improved biotinylation... England BioLabs). MBP has a similar function as GST in that it provides a method to express a foreign...

...proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant proteins for ...purification; has inherent enterokinase cleavage site GST Large Protein 26 Purification and Stability Good ...

...tagging at either the N- or C-terminus (3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary...

...cloning vector (8A)) and have an epitope tag (6xHis or GST) to affinity purify the expressed protein with a ...

...concentration of inducer or adding an affinity tag such as GST may help with solubility issues. We want to hear...

... tags (including His, S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA and SUMO) for optimizing expression...

...each day. This requires tremendous coordination amongst the lab team, shipping team, and others to ensure...contributions on social media as well the excitement amongst our Addgenies on social media or around the office...

...at the time, got involved. Along with Philipp Pfingstag, a visiting graduate student from TU München at...  References 1. Kunjapur, Aditya M., Philipp Pfingstag, and Neil C. Thompson. "Gene synthesis allows ...

...is one of the leaders in distributing plasmids amongst the academic community. On the BioBricks Foundation...have two types of languages: one to communicate amongst each other, like this conversation we are having...

...broader issues in this brief volume. He examines, amongst other topics: racism and sexism in science, problems...originally isolated the GFP gene but was not included amongst the Nobel prize winners for developing the technology...

...that makes them difficult to approach for the youngster just starting in the lab, but they are exactly...

...scientific conference, you may think about sitting amongst a sea of chairs listening to talks all day. But...

...wanted to run and play with my kids like any other youngster. His parents, Kristi and Rico, had to keep him...

...to promote interest in the biological sciences amongst the student community at the the University of ...

...it hard to stick to vertebrates alone.  And a longstanding inclination to be a teacher was looking for ...

...research is totally dependent on communication amongst the team so getting everyone talking to each other...

...career ambitions. First, how does one stand out amongst a pool of successful applicants and get chosen ...

...Sharon Dent Archa Fox Elaine Fuchs Kai Ge Robert Kingston Thoru Pederson Phil Sharp Julie Ahringer Alexander...

...specific genes and an immune response has been a longstanding challenge. Using shRNA, TALEN, or ZFN to edit...