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  1. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...western blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated...being purified by GST (maybe try MBP if you are having solubility issues!). Need a GST antibody? Addgene...sites immediately following the tag. Unlike MBP, the GST tag can be cleaved off from the fusion protein while...a handy feature. If you decide not to cleave the GST tag after/during purification, be aware that the ... affect downstream applications. Also, purifying GST by affinity chromatography requires proper folding...

  2. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on a reduced...immobilized target protein. The disadvantage is that the GST moiety, which forms dimers, may influence binding...experimental protein-ligand interaction approaches that of GST-glutathione. Biotin-based immobilization for kinetic...avidin can be controlled easier than formation of GST dimers. Avi-Tag plasmid for improved biotinylation... England BioLabs). MBP has a similar function as GST in that it provides a method to express a foreign...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...expression vector with N-terminal GST pGCS-N5(GST) - GST tagged Gateway destination vector for mammalian...C-terminal mTagBFP2 tag pNIC-GST-TEV-GG - Cloning of target gene with N-terminal GST tag and TEV cleavage Transient...myristoylation signal GST Protein purification pEBG - N-terminal GST for mammalian...mammalian expression pET His6 GST TEV - N-terminal His6-GST-TEV for bacterial ... of an N-terminal GST tag fusion with your gene of interest pFastbac1 Nterminal GST - Baculovirus expression...destination vector for bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal 3x...expression pDest-565 - N-terminal His6-GST for bacterial expression (Gateway) pE4n - Modified...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Gitler 29629 GST-TEV-FUS FUS TEV, GST T7 ALS Aaron Gitler 29630 GST-TEV-FUS-267-526aa FUS TEV, GST T7 ALS Aaron...29631 GST-TEV-FUS-1-373aa FUS TEV, GST T7 ALS Aaron Gitler 29632 GST-TEV-FUS-1-413aa FUS TEV, GST T7 ALS...Marc Timmers 44978 GST-FUS-WT FUS GST tac ALS Robin Reed 44981 GST-FUS 266-526 FUS GST tac ALS Robin Reed...Reed 44982 GST-FUS 356-526 FUS GST tac ALS Robin Reed 44983 GST-FUS 465-526 FUS GST tac ALS Robin Reed ...666) TBK1 GFP, GST, Tev P10 ALS Sascha Martens 198428 pGST2-GST-TEV-OPTN OPTN TEV, GST tac ALS Sascha ...) ADAM10 GST CMV Alzheimer's Haian Fu 189562 pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GST ADAM10 GST CMV Alzheimer's...187827 pGST2-GST-TEV-OPTN S177D S473D OPTN GST-TEV ALS Sascha Martens 187830 pFastBac_Dual_GST-TEV-EGFP-TBK1...

  5. Plasmids 101: Protein tags

    Type
    Blog Post
    ...proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant proteins for ...purification; has inherent enterokinase cleavage site GST Large Protein 26 Purification and Stability Good ...

  9. Bacterial Expression Systems

    Type
    Collection
    ...SGC 39188 pTac (lactose/IPTG inducible) N terminal GST tag with TEV cleavage site to remove tag Nicola Burgess...Burgess Brown pGEX expression vector with N-terminal GST tag and TEV cleavage site. Includes sites for LIC...combinations of N and C-terminal Strep, His, MBP, TRX, GST, SUMO, MISTIC, and LSL tags as well as tags for protein...

  11. Zebrafish Plasmid Collection

    Type
    Collection
    ...carboxyl-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible...

  14. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...25R] PSD-95 Human Mouse IgG2a 188214 Anti-GST [N100/13R ] GST Schistosoma japonicum Mouse IgG2a 188215 ...N1/12 scFv KCC2 Rat Mouse 190521 GST scFv [N100/13] N100/13 scFv GST Schistosoma japonicum Mouse 190522...

  16. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    ...each day. This requires tremendous coordination amongst the lab team, shipping team, and others to ensure...contributions on social media as well the excitement amongst our Addgenies on social media or around the office...

  20. AAV Production in HEK293 Cells

    Type
    Protocol
    ...and forth. Pro-Tip To help distribute the media amongst the five layers, tilt the CS5 such that the media...
Showing: 1 - 20 of 47 results