We narrowed to 7 results for: KLF4

1. 15 Hot Plasmids from 2017

   Type

   Blog Post

   Published

   Jan. 9, 2018, 3:02 p.m.

   ...variation in the Klf4 isoform (KLF4s or KLF4L). Polycistronic cassettes that contained KLF4s had overall diminished...Gateway-Compatible Toolbox Podcast Segment The impact of KLF4 N-terminal variants on iPSC generation Deriving ...expression of transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model for reprogramming... diminished KLF4 expression levels; this altered stoichiometry impacted reprogramming and global gene ... monocistronic expression of either variation of Klf4 did not lead to the observed reprogramming discrepancies...available at Addgene and include a variety of cMYC, KLF4, SOX2 and OCT4 DNA binding domain and transcription...patterns. Polycistronic cassettes that contained the KLF4L isoform had more robust initiation of reprogramming...

2. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ...expression of the reprogramming factors- OCT3/4, SOX2, KLF4, and C-MYC [1]. For the derivation of clinical grade...vectors that encoded six factors - OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen (SV40LT)....Plasmid Addgene ID# Encodes pCXLE-hSK 27078 SOX2 and KLF4 pCXLE-hUL 27080 L-MYC and LIN28 pCXLE-hOCT3/...

3. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

   Type

   Blog Post

   Published

   Sept. 3, 2019, 1:15 p.m.

   .... When the group targeted the enhancer region of Klf4 and the promoter region of Zfp462, which are over...

4. Four Factors that Differentiate the Stem Cell Field

   Type

   Blog Post

   Published

   Sept. 28, 2016, 2:35 p.m.

   ...cells simply by expressing four proteins Oct4, Sox2, Klf4, and cMyc (the so called OSKM factors). As the Yamanaka...

5. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

   Type

   Blog Post

   Published

   Oct. 30, 2018, 1:04 p.m.

   ... expression of four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated from mouse...

6. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

   Type

   Blog Post

   Published

   Nov. 6, 2018, 1:12 p.m.

   ...overexpression of four reprogramming factors- OCT4, SOX2, KLF4, and C-MYC - that induce a differentiated somatic...

7. Delivery Methods for Generating iPSCs

   Type

   Blog Post

   Published

   April 17, 2018, 1:37 p.m.

   ... reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids if you’re interested...