...Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for targeted...For lentiviral expression, Fuw-dCas9-Dnmt3a and Fuw-dCas9-Dnmt3a-P2A-tagBFP are available from Rudolf ...ready-to-use lentivirus. Figure 3: dCas9-DNMT3A methylates cytosines at promoters and enhancers...transient and quickly reversed in culture. However, DNMT3A-induced methylation persisted throughout a 100 ...

...acetyltransferase p300 or the DNA methyltransferase DNMT3A allows researchers to easily add biochemical marks...Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3 activity in pluripotent cells at thousands of methylated...

...   Figure 4: CHARM uses a fusion of the DNMT3L cofactor (D3L) with a histone tail (H3K4me0) to...the endogenous, autoinhibited methyltransferase DNMT3A to the genomic location specified by the DNA-binding...

... demethylation by LSD1, cytosine methylation by DNMT3A or MQ1, and cytosine demethylation by Tet1. RNA...

...the catalytic domain from DNA methyltransferase, DNMT3A. Co-expression of this modified Cas9 along with...