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Showing: 1 - 14 of 14 results

  1. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for targeted...For lentiviral expression, Fuw-dCas9-Dnmt3a and Fuw-dCas9-Dnmt3a-P2A-tagBFP are available from Rudolf ...ready-to-use lentivirus. Figure 3: dCas9-DNMT3A methylates cytosines at promoters and enhancers...transient and quickly reversed in culture. However, DNMT3A-induced methylation persisted throughout a 100 ...

  3. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...   Figure 4: CHARM uses a fusion of the DNMT3L cofactor (D3L) with a histone tail (H3K4me0) to...the endogenous, autoinhibited methyltransferase DNMT3A to the genomic location specified by the DNA-binding...

  5. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...the catalytic domain from DNA methyltransferase, DNMT3A. Co-expression of this modified Cas9 along with...

  6. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... pdCas9-DNMT3A-EGFP 71666 Mammalian U6 yes, methylation S. pyogenes EGFP Zoldos pdCas9-DNMT3A-PuroR 71667...pCBC-MT2T3 50594 Plant BsaI none S. pyogenes Chen pCBC-MT3T4 50595 Plant BsaI none S. pyogenes Chen pBUN421 62204...Mammalian S. pyogenes Neo, mCherry Postovit pdCas9-DNMT3A-PuroR_v2 74407 Mammalian U6 yes, methylation S....

  7. CRISPR Plasmids - Tagging

    Type
    Collection
    ...PX458_ZNF580_1 PX458_ZNF580_2 DNMT3B Human FLAG pFETCh_DNMT3B PX458_DNMT3B ZNF219 Human FLAG pFETCh_ZNF219...

  8. Validated gRNA Sequences

    Type
    Collection
    ...Sabatini DNMT3A H. sapiens GAGATGATCGCCCCTTCTTC 41822 cut S. pyogenes 23287722 Church DNMT3A H. sapiens...GCATGATGCGCGGCCCAAGG 41821 cut S. pyogenes 23287722 Church DNMT3B H. sapiens GAATTACTCACGCCCCAAGG 41823 cut S. pyogenes...

  9. CRISPR Guide

    Type
    Collection
    ... is an all-in-one dCas9 fusion with KRAB, DNMT3A, and DNMT3L. CRISPRoff maintains gene silencing across...TET1 - activation through cytosine demethylation DNMT3A - repression through cytosine methylation MQ1 -...

  10. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...histone demethylation by LSD1 cytosine methylation by DNMT3A or MQ1 cytosine demethylation by Tet1 These modifications...

  12. CRISPR Plasmids - Plants

    Type
    Collection
    ...see paper BsaI none S. pyogenes Chen 50595 pCBC-MT3T4 see paper BsaI none S. pyogenes Chen 62204 pBUN421...

  13. CRISPR Guide

    Type
    Guide
    ... is an all-in-one dCas9 fusion with KRAB, DNMT3A, and DNMT3L. CRISPRoff maintains gene silencing across...TET1 - activation through cytosine demethylation DNMT3A - repression through cytosine methylation MQ1 -...

  14. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...] Malin Human Mouse IgG2a 114514 Anti-DNMT3L [N117/9.5R] DNMT3L Human Mouse IgG2a 114515 Anti-GABA(A)R...
Showing: 1 - 14 of 14 results