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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9 results for: PLS

Showing: 1 - 9 of 9 results

  1. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ... structures. Enzyme-catalyzed proximity labeling (PL) uses a promiscuous labeling enzyme and a small-molecule...Until recently, the enzymes most frequently used for PL were APEX2 and BioID. APEX2 is fast, tagging proximal...increased efficiency of TurboID and miniTurbo makes PL a viable method for proteomics in model organisms...

  2. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...tightly regulated by the endogenous phage promoter pL and repressor CI. For recombineering purposes, a ...-inducible pBAD promoter and the endogenous phage pL promoter.  It is a good idea to choose plasmids that... genome.  Mini-λ uses the endogenous red promoter pL and the cI857 repressor to regulate expression. An...

  5. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...10.2174/1566523054546224 DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL, Jolly DJ, Dubensky TW Jr ...

  6. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ... (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange Mammalian Express a gene of interest...interest fused to the N-terminus of LSSmOrange pLSSmOrange-C1 Orange Mammalian Express a gene of interest...

  8. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...biology’, Frontiers in Plant Science, 4. doi: 10.3389/fpls.2013.00339. PubMed PMID: 24032037. PubMed Central...
Showing: 1 - 9 of 9 results