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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15 results for: PLS

Showing: 1 - 15 of 15 results

  2. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...tightly regulated by the endogenous phage promoter pL and repressor CI. For recombineering purposes, a ...-inducible pBAD promoter and the endogenous phage pL promoter.  It is a good idea to choose plasmids that... genome.  Mini-λ uses the endogenous red promoter pL and the cI857 repressor to regulate expression. An...

  5. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Puro Ebert pL-CRISPR.EFS.PAC 57828 Mammalian/Lentiviral BsmBI yes, cut S. pyogenes Puro Ebert pL-CRISPR.SFFV.PAC...Mammalian/Lentiviral BsmBI none S. pyogenes Zhang pL-CRISPR.EFS.GFP 57818 Mammalian/Lentiviral BsmBI yes...Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pL-CRISPR.EFS.tRFP 57819 Mammalian/Lentiviral BsmBI ...Lentiviral BsmBI none S. pyogenes tagRFP657 Ebert pL-CRISPR.SFFV.GFP 57827 Mammalian/Lentiviral BsmBI ...Mammalian/Lentiviral BsmBI none S. pyogenes tagRFP Ebert pL-CRISPR.SFFV.tRFP 57826 Mammalian/Lentiviral BsmBI...Mammalian/Lentiviral none S. pyogenes Puro Weissman pLSC-5 62889 Mammalian/Lentiviral BsmBI yes, cut (split...

  6. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...10.2174/1566523054546224 DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL, Jolly DJ, Dubensky TW Jr ...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...fusions using Gateway cloning Mikko Taipale 106253 pLS-mP-Luc Firefly Insertion of 5' promoter/enhancer ...regions controlling transcription Pete Stecha 106292 pLS-SV40-mP-Rluc Renilla Insertion of 5' promoter/enhancer...

  8. Sequencing Primers

    Type
    Guide
    ...ACTGAGCACATCAGCAGGAC Lambda phage early leftward (pL) promoter, forward primer pLXSN 5' CCCTTGAACCTCCTCGTTCGACC...

  9. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ... (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange Mammalian Express a gene of interest...interest fused to the N-terminus of LSSmOrange pLSSmOrange-C1 Orange Mammalian Express a gene of interest...

  13. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... 572 23 5.7 2.3 h Monomer pLSSmOrange-C1 - Mammalian Expression pLSSmOrange-N1 - Mammalian Expression ...624 2.5 3.2 100 min Monomer pLSSmKate1-N1 - Mammalian Expression pLSSmKate1-C1 - Mammalian Expression pBAD...Expression LSSmKate2 460 605 4.4 2.7 2.5 h Monomer pLSSmKate2-N1 - Mammalian Expression pBAD/HisD-LSSmKate2...

  14. Fluorescent Proteins: FRET

    Type
    Collection
    ....45 633 62,500 0.40 5.6 3.7 pLSSmOrange-N1 , mKate1.31-pBAD , pLSSmOrange-mKate2 LSSmOrange* mScarlet3...mScarlet3 437 0.45 592 104,000 0.75 5.8 4.3 pLSSmOrange-N1 , pmScarlet3_C1 mT-Sapphire* tdTomato 399 0.6 581 ...
Showing: 1 - 15 of 15 results