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We narrowed to 6 results for: PX330

Showing: 1 - 6 of 6 results

  1. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...as the second design (in pX330/PX335), so we recommend people all use the pX330/PX335 system. Additionally...the oligo when cloning my target spacers into the PX330 vector backbone? A2: There is no need to add the...having a targeting spacer cloned into the backbone (pX330 or PX335, depending if you want to use wildtype ...wildtype Cas9, clone two spacers for each locus into PX330, then co-transfect or delivery the two plasmids ...not a very big concern, you could go ahead with PX330 plasmid, clone in your target guides, test run them...into the cells with the Cas9/sgRNAs vector (like PX330). For large changes (>100 bp insertions or deletions...if the cloning of my oligo into a backbone like PX330 worked? A12: You can quickly check which of your...

  2. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...expression cassette. (b) pCAG-EGxxFP target plasmid and pX330-sgRNA plasmid (Mashiko et al. 2013). The pCAG-EGxxFP...-cloning site (MCS) between EGFP fragments. The pX330 plasmid contains humanized Cas9 expression cassette...pCAG-EGxxFP target plasmid was co-transfected with pX330 plasmids containing sgRNA sequences into HEK293T...plasmid along with the individual gRNAs in their PX330 plasmids (or similar plasmids for gRNA expression...system. However, it is easier to directly inject the pX330 plasmids used in the validation assay (above) and...

  3. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...gRNAs into spectinomycin-resistant plasmids pX330S-2 to pX330S-(last gRNA number). The 5’ most gRNA is cloned...gRNA from a single promoter using a plasmid like pX330? Unfortunately, the short answer is no. Unless you... from the Ventura lab. pX333, a modification of pX330, contains humanized wtCas9 and two U6 promoters....expressing 4 gRNAs, you’d use pX330A-1x4; for 6 gRNAs, you’d use pX330A-1x6. This customization means ...

  4. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...over the years on their own or through Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9... be cloned to create a chimeric guide RNA. Find pX330-U6-Chimeric_BB-CBh-hSpCas9. pSpCas9(BB)-2A-Puro ...

  5. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...sequence into a Cas9-containing plasmid such as pX330. This cloning strategy not only makes it easy to...
Showing: 1 - 6 of 6 results