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We narrowed to 6 results for: PX330

Showing: 1 - 6 of 6 results

  1. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...we discuss the usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows...guide’s reverse complement for cloning into the pX330 vector using BbsI restriction enzyme ( Table 3 )...G. sgRNA expression from the U6 promoter of the pX330 vector is enhanced by the inclusion of a G nucleotide...concentration of 1 μM). Ligate annealed oligos into pX330 using a Golden Gate assembly cloning strategy 3 .... Prepare a 50 μl reaction mix: 100 ng circular pX330 vector, 1.0 μl annealed oligos (1 μM; see step 3.1.4...

  2. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Yamamoto Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9...all-in-one CRISPR/Cas9 vector based on the Zhang lab's PX330 , simultaneously targeting the genomic site and ...gene-specific CRISPR and donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the...

  3. CRISPR References and Information

    Type
    Collection
    ... Deletions in Mammalian Cell Lines pSpCas9(BB) (pX330) Protocol at Addgene Parrott NEBuilder Assembly ... (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) PDF, 255 KB Zhang sgRNA cloning...

  4. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Mammalian BbsI yes, nick S. pyogenes Puro Zhang PX330 (2nd Gen) 42230 Mammalian BbsI yes, cut S. pyogenes...Mammalian U6 yes, methylation S. pyogenes Puro Zoldos pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110) 71707 Mammalian...

  5. Zhang Lab CRISPR Page

    Type
    Collection
    ...plasmids can be ordered via the links below: 42230 : PX330; SpCas9 and single guide RNA 48138 : PX458; SpCas9...
Showing: 1 - 6 of 6 results