We narrowed to 11 results for: Psp

...lentiGuide-Puro. pSpCas9n(BB)-2A-GFP (PX461) - Similar to pSpCas9(BB)-2A-Puro (PX458), pSpCas9n(BB)-2A-GFP ...The top 15 plasmids requested from Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid... sequence (pSpCas9(BB)). The 2A-GFP allows for screening of transfected cells. Find pSpCas9(BB)-2A-GFP...nick, rather than cleave DNA. Find pSpCas9n(BB)-2A-GFP (PX461). pSpCas9(BB)-2A-Puro (PX459) - This mammalian...so it may be best to go with pSpCas9(BB)-2A-Puro (PX459) V2.0. Find pSpCas9(BB)-2A-Puro (PX459). Thank...most requested plasmid of all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2...the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (PX458) - This plasmid from the Zhang...

...initial tests, Cas13b from Prevotella sep. P5-125 (PspCas13b) yielded 62.9% average knockdown, and they chose...chose this enzyme for further studies. PspCas13b does not require a PFS, and it is sensitive to mismatches...They fused ADAR deaminase domains (ADARDD) to dPspCas13b, but observed low RNA editing. To increase A-...template has a cytosine mismatch at that position. PspCas13b-ADAR2DD(E488Q) displayed robust editing with various...showed that editing is mediated by ADAR2DD, not PspCas13b. Find the plasmids for the RESCUE RNA editors ...

...further ado, the most request plasmid in 2016 was... pSpCas9(BB)-2A-Puro (PX459) V2.0       Similar to last... information). V2.0 This designation separates pSpCas9(BB)-2A-Puro (PX459) V2.0 from it's predecessor,...This point mutation has been corrected in V2.0. pSpCas9(BB)-2A-Puro (PX459) V2.0: What's not in the name...

...postsynaptic potentials (mEPSPs) (Hao and Lee et al., 2024). While studying mEPSPs has been possible with...difficult with GEVIs. Not only was ASAP5 able to detect mEPSPs, it also enabled imaging of their propagation along...

... and PE5 prime editing strategies) based on a DeepSpCas9 score ranking (Kim et al., 2019). PRIDICT saves..., H. H. (2019). SpCas9 activity prediction by DeepSpCas9, a deep learning–based model with high generalization...

...packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two plasmids from Didier Trono's...

...lentiviral production and packaging vectors VSV-G and psPAX2, which were used in a publication by Xiuyuan Ou...

...can also be combined in single-plasmid INTEGRATE (pSPIN), which offer simple transformations for smaller...

...produced in 293T cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono...

...integrase-deficient packaging plasmid available at Addgene: psPAX2-D64V. This construct has the D64V mutation, a single...

... HEK293T cells with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260). HeLa cells were infected with...