...endogenous TRIM21 is sufficient for TRIM-Away, and you only need to deliver the target antibody. TRIM-Away ... proteasome function. TRIM-away compared favorably to drug inhibition, with TRIM-away targeting of mTOR...hope they will TRIM down the difficulty of your protein knockdown experiments! The TRIM-Away plasmids ...antibody. TRIM21, an E3 ubiquitin ligase, is the basis of TRIM-away. In normal physiology, TRIM21 recruits... methods for TRIM-Away delivery. For small cell populations, co-microinjection of TRIM21 protein and the...time to read? Listen to our Hot Plasmids Podcast! TRIM-Away: Targeted endogenous protein degradation Article...Article contributed by Mary Gearing Listen to the TRIM-Away podcast segment CRISPR and RNAi have helped...

... developed for a singular purpose (ex. adapter trimming, quality score analysis, k-mer counting, or sequence...process. For example, our bbduk process created trimmed FASTQ files as output, which we specified as input...

...Alex hopes to help Addgene advance - plants for trimmed down protocols, distribution of new materials (...

... of telomere length regulation called telomere trimming and have proposed a role for TZAP, a Kruppel-like...regulating the upper-bound of telomere length. Telomere trimming can be assayed by looking for the production of...

...validation of some of these tools created by the Trimmer Lab that will be the first antibodies distributed...constructing the plasmids for expression of mAbs, the Trimmer lab isolated RNA from cryopreserved hybridomas,...can be a threat to future reproducibility. The Trimmer Lab found that in addition to recovering RNA from...lab’s entire hybridoma collection. Instead, the Trimmer Lab moved to direct functional validation by immunofluorescence-immunocytochemistry...subsequently cloned into the expression constructs. The Trimmer lab found that the recognition sequence for the...webpage. Importantly, the methods developed by the Trimmer Lab for construction and validation of R-mAb expressing...with different mAbs in the same experiment. The Trimmer Lab found that they could modify the CH encoding...

...James Trimmer of the University of California Davis School of Medicine on this grant. Dr. Trimmer has created... ready-to-use-proteins to facilitate use. Dr. Trimmer’s lab will ensure the quality of the antibodies ...Addgene to bring this resource to life,” says Dr. Trimmer. But in order to create such a resource, we need...Initiative has been funding laboratories, such as Dr. Trimmer’s lab, to create recombinant antibodies for use ..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...

...coupled with heterotrimeric G proteins on the intracellular side of the cell membrane. Heterotrimeric G proteins...in signal transduction pathways and consist of a trimer of three subunits: alpha (Gα), beta (Gβ), and gamma...the GPCR allows for binding of a new inactive heterotrimeric G protein complex and subsequently another ...elucidate the role of 14 of the 16 non-visual heterotrimeric G proteins that can be activated downstream... alpha, 2 beta, and 4 gamma subunits of the heterotrimeric G protein complex that can be used to detect...

... al., 2020). A transducer complex consists of a trimer of three subunits: alpha, beta, and gamma (Gα, ..., Gβ, and Gγ), collectively known as a heterotrimeric G protein. This complex converts a signal received.... When inactive, these subunits are found in a trimeric complex bound to the intracellular surface of ...called BRET2. Figure 1: TRUPATH measures heterotrimeric G protein dissociation by bioluminescence resonance...

...presence of trimethoprim, cultures treated with a folA gRNA were more sensitive to trimethoprim when compared... which encodes the target for the antibiotic trimethoprim. When Enterobacter aerogenes, Klebsiella pneumoniae... the folA gene product not been a target for trimethoprim, there would be no changes in sensitivity. Pooled...

...antibodies available at launch were created by James Trimmer, PhD, of the University of California Davis School... Figure 1: Antibody against PSD-95 from the Trimmer Lab. Information about the antibody, the target...Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant...

...Bendezú et al., 2015) and the alpha subunit of heterotrimeric G-proteins (Janetopoulos et al., 2001, Adjobo-Hermans...Merel JW, et al. "Real-time visualization of heterotrimeric G protein Gq activation in living cells." BMC...Devreotes. "Receptor-mediated activation of heterotrimeric G-proteins in living cells." Science 291.5512...

...with fluorescent derivatives of its substrate trimpethoprim (TMP-tag) (Miller et al., 2005). Although this...Benzylguanine (BG) Many Easy eDHFR 159 Trimpethoprim  (TMP-tag) Few Easy HaloTag 295 ... V. W. (2005). In vivo protein labeling with trimethoprim conjugates: A flexible chemical tag. Nature ...

...Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...

...reliable expression, and easy distribution as DNA (Trimmer, 2020), you may be interested in using them in ...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...

...anti-GFAP [N206A/8R] antibody shared by James Trimmer’s lab (Figure 5) as well as antibodies for identifying...N206A/8 TC Supernatant). Image attribution: James Trimmer, UC Davis.   ...

...have successfully generated several functional heterotrimeric G-alpha subunit fusions that do not tolerate...Merel JW, et al. "Real-time visualization of heterotrimeric G protein Gq activation in living cells." BMC...

...IF labeling of rat brain samples from the James Trimmer Lab, using recombinant antibodies with different...Der List, D., Murray, K. D., Engebrecht, J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched...

...In contrast, Flag and HA tags had especially detrimental effects on cell size and growth rate, indicating...

...Errington, T. M., Turn, R. E., Bandrowski, A., Trimmer, J. S., Rego, M., Freedman, L. P., Ferrara, F.,...

..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...