We narrowed to 7 results for: Tpr;
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TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m....first-generation mARGs also had a large genetic footprint (two cassettes + a booster cassette), their expression...mARGs with higher expression, a smaller genetic footprint, and nonlinear acoustic scattering. The best candidate... Increasing expression and decreasing genetic footprint We took a cue from the native expression patterns...cassette, either, allowing us to reduce the genetic footprint yet again. This also led to improved expression...
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PiggyBac-ing Through the Genome Editing Field
TypeBlog PostPublishedMay 31, 2016, 3:30 p.m....transposon gene modification system and the scarless, Footprint-Free™ removal of cargo with the excision-only ...thereby leaving behind only those cells with a Footprint-Free™ genome edit. Figure 2: Editing...nuclease to stimulate homologous recombination and Footprint-Free™ removal of the selection cassette by excision-only... -
Plasmids 101: Using Transposons in the Lab
TypeBlog PostPublishedJuly 6, 2021, 1:28 p.m....excises the transposon from the donor DNA. A DNA footprint is left behind. When the transposon-transposase...dinucleotides and it leaves behind the CAG DNA footprint from its terminal sequences at the excision site...other transposons, it does not leave behind a DNA footprint sequence after excision. piggyBac can mobilize... -
Delivery Methods for Generating iPSCs
TypeBlog PostPublishedApril 17, 2018, 1:37 p.m..... Viral vectors also have the largest genomic footprint of iPSC generating methods due to the risk of ...Non-integrating methods have a smaller genetic footprint compared to integrating approaches. These methods... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....Genome-wide tracking of dCas9-methyltransferase footprints. Nat Commun 9, 597. https://doi.org/10.1038/s41467... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....editing efficiency and reduced RNA-off target footprint compared to current N-terminal linked SaCas9 ABE... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....kits, but you’d like to reduce your lab’s waste footprint. The Zhao and Deng labs developed a simple method...