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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 16 results for: U6

Showing: 1 - 16 of 16 results

  1. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...slightly lower expression than U6. May have better expression in neuronal cells. U6 small RNA expression shRNA...shRNA From the human U6 small nuclear promoter Constitutive  Murine U6 is also used, but may be less efficient...

  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...the years on their own or through Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9...cloned to create a chimeric guide RNA. Find pX330-U6-Chimeric_BB-CBh-hSpCas9. pSpCas9(BB)-2A-Puro (PX459...chimeric RNA element with customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a ...

  3. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...target sequence downstream of the 7SK, human U6, mouse U6, or human H1 promoters. If you express fewer...modification of pX330, contains humanized wtCas9 and two U6 promoters. To use this plasmid, you simply order ...and up to three gRNAs. Entry vectors containing the U6 promoter and the gRNA scaffold are provided with ... cloning. gRNAs are expressed from two Drosophila U6 promoters. Cas9 must be supplied on a separate plasmid...

  4. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ... first site accepts the sgRNA target sequence for U6 promoter expression and the second site accepts the...containing a direct fusion of the desired gRNA to the U6 promoter, and a second plasmid containing the homology...

  5. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ... upstream of the Cbh promoter, this plasmid has a U6 promoter followed by BbsI restriction sites into ...

  7. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...strategy, the need for lengthy promoters, such as U6 or EF1α promoters, has limited the number of gRNAs...

  8. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...target sequence into a backbone that uses the human U6 promoter to drive expression, is it necessary to ...to the start of my target sequence? A3: The human U6 promoter prefers a 'G' at the transcription start...

  9. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...Libraries express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and dual-targeting hybrid gRNA...

  12. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...uniquely transcribed by RNA Polymerase III (5S, tRNA and U6) and measuring Corn fluorescence by flow cytometry...

  14. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive (H1, U6, 7SK) or inducible shRNA-miR30...of the available sgRNA-cargo constructs utilize a U6 promoter and contain the RNA cargo cassette inserted...

  15. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...ES) cells. These vectors, termed RUSH (For ROSA26 U6 short hairpin) and CRUSH (Conditional RUSH) use Cre-mediated...

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter. Addgene...
Showing: 1 - 16 of 16 results