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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 33 results for: U6

Showing: 1 - 20 of 33 results

  1. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...slightly lower expression than U6. May have better expression in neuronal cells. U6 small RNA expression shRNA...shRNA From the human U6 small nuclear promoter Constitutive  Murine U6 is also used, but may be less efficient...

  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...the years on their own or through Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9...cloned to create a chimeric guide RNA. Find pX330-U6-Chimeric_BB-CBh-hSpCas9. pSpCas9(BB)-2A-Puro (PX459...chimeric RNA element with customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a ...

  3. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...target sequence downstream of the 7SK, human U6, mouse U6, or human H1 promoters. If you express fewer...modification of pX330, contains humanized wtCas9 and two U6 promoters. To use this plasmid, you simply order ...and up to three gRNAs. Entry vectors containing the U6 promoter and the gRNA scaffold are provided with ... cloning. gRNAs are expressed from two Drosophila U6 promoters. Cas9 must be supplied on a separate plasmid...

  4. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ... first site accepts the sgRNA target sequence for U6 promoter expression and the second site accepts the...containing a direct fusion of the desired gRNA to the U6 promoter, and a second plasmid containing the homology...

  7. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 74009 Zebrafish U6 yes, cut S. pyogenes EGFP Del Bene pUAS:Cas9T2ACre;U6:sgRNA1... Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868 Mammalian U6 yes, cut N. meningitidis...LEU2 Lu pDestTol2CG2-U6:gRNA 63156 Zebrafish BseRI none S. pyogenes Zon pDestTol2pA2-U6:gRNA 63157 Zebrafish...pSmart-Nm-sgRNA-BbsI 49157 Mammalian U6 none N. meningitidis Thomson pXCas9H840A 60900 Mammalian U6 yes, nick S. pyogenes...Mammalian/Lentiviral U6 none S. pyogenes Pederson pLH-nmsgRNA1.1 64115 Mammalian/Lentiviral U6 none N. meningitidis.../Lentiviral U6 none N. meningitidis Pederson pLH-stsgRNA2.1 64117 Mammalian/Lentiviral U6 none N. meningitidis...Lentiviral U6 none N. meningitidis Pederson pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6 64220 Mammalian U6 yes,...

  9. Zhang Lab CRISPR Page

    Type
    Collection
    ...are described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR...PX601; CMV-driven SaCas9; U6-driven sgRNA 61593 : PX602; TBG-driven SaCas9; U6-driven sgRNA 61592 : PX600...promoter-driven SpCas9; for neuronal expression 60958 : PX552; U6 promoter-driven; for sgRNA cloning; has GFP-KASH ...binding globulin (TBG, PX602 [#61593] ) promoter, and a U6-driven single guide RNA. The vector can be digested...acceptor: The PX552 plasmid (#60958) contains pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor)... directed repair donor template. #60225 - AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This ...is used as a control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This...

  10. CRISPR Plasmids - Plants

    Type
    Collection
    ...53062 pU6-gRNA wheat U6 BbsI none S. pyogenes Gao 59188 pBlu/gRNA Arabidopsis U6 BbsI none S. pyogenes...pyogenes Hyg Chen 78098 pHAtC U6 AarI yes, cut S. pyogenes Hygro Kim 78097 pBAtC U6 AarI yes, cut S. pyogenes...Basta Kim 71286 pHEE401 U6-26p BsaI yes, cut S. pyogenes Hygro Chen 71287 pHEE401E U6-26p BsaI yes, cut S....aU6 BsaI none S. pyogenes Kamoun 52255 pUC119-gRNA U6 PCR template none S. pyogenes Sheen 51295 pRGEB31...pyogenes Hygro Chen 78932 pHDE-35S-Cas9-mCherry-UBQ U6-26 PmeI yes, cut S. pyogenes Hygro, mCherry Zhao ...

  11. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...target sequence into a backbone that uses the human U6 promoter to drive expression, is it necessary to ...to the start of my target sequence? A3: The human U6 promoter prefers a 'G' at the transcription start...

  12. Hot Plasmids - October 2020

    Type
    Blog Post
    ...Libraries express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and dual-targeting hybrid gRNA...

  14. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Drosophila U6:3 pegRNA BbsI No Vermilion Norbert Perrimon 149546 pCFD5-NS Drosophila Drosophila U6:3 pegRNA...Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI for sgRNAs; BbsI + PstI...Mammalian hU6 epegRNA BsaI No mRFP1 David Liu 176901 U6-pe RNA-H1-nick sgRNA-mCherry Mammalian...

  15. Lentivirus Plasmids

    Type
    Collection
    ... 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning Root 14748 pLKO.3G 3rd U6-driven...Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro resistance. ...Trono 11795 pLL3.7 3rd Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included ...silencing were added; Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included ...

  17. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...reporters via an H2B fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids...shuttle plasmids to insert a second guide within a U6-guide cassette. This process is compatible with all...

  18. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Yildiz 159787 pGL3-U6-pegRNA_KCNA1-EGFP KCNA1 U6 Episodic ataxia Xiaolong Wang 159788 pGL3-U6-sgRNA_KCNA1-mcherry...190900 pAAV2ss-U6-sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51-7sk-...7sk-Cas9 HTT U6, 7sk Huntington's Nicole Deglon 190902 pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1 HTT GFP U6, EFS Huntington's...Deglon 190903 pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9 HTT GFP U6, EFS Huntington's Nicole Deglon 191487...78622 pX335_HR_Prnp_3 PRNP U6 Dementia Walker Jackson 78623 pX459_HR_Prnp_3 PRNP U6 Dementia Walker Jackson...MS2)_Prnp_SAM1 PRNP U6 Dementia Walker Jackson 78625 sgRNA(MS2)_Prnp_SAM2 PRNP U6 Dementia Walker Jackson...SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS Rajat Rohatgi...

  20. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...none, need Cas9 plasmid Bullock and Port 49411 pCFD4-U6:1_U6:3tandemgRNAs dU6:1 and dU6:3 BbsI Injection ...Virmilion none, need Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 ...
Showing: 1 - 20 of 33 results