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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 61 results for: UNK

Showing: 1 - 20 of 61 results

  1. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...on the expected concentration of the unknown sample. The unknown should fall somewhere in the middle of...determine the concentration of a target molecule in an unknown sample. To do this, you serially dilute a control...information to extrapolate the concentration of an unknown sample. For example, a BCA assay uses a serial ...dilution of a control protein, incubated alongside an unknown sample with the BCA reagent. The absorbance of ...allows you to determine the concentration of the unknown sample.  How do I make the standards for a standard... prepare the standard, standard dilutions, and unknown sample(s) in the same buffer if possible, or very..., you can extrapolate the concentration of the unknown sample based on its measured response. An example...

  2. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...Mice Unknown Kumar et al., 2020 AAV-PHP.AX CNS: neurons, astrocytes Mice Unknown Jang ... ganglion neurons Mice, rats No (mechanism unknown) Chan et al., 2017 Asencor et al., 2022 AAV-PHP.eC... Mice, rats, rhesus macaques No (mechanism unknown)   Chen et al., 2023 AAV1-X1 Brain endothelial...endothelial cells Mice No (mechanism unknown)   Chen et al., 2023 AAV9.452sub.LUNG1 Lung: ATII...CNS: neurons and astrocytes Mice, marmosets Unknown Goertsen, Flytzanis, et al., 2022 AAV.CAP-B10...inhibitory and excitatory neurons Mice, marmosets Unknown Brown et al, 2021 Goertsen, Flytzanis, et al....endothelial cells Mice, rats No (mechanism unknown) Krolak et al., 2022 AAV.MaCPNS1 PNS (mice...

  3. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...activate many GPCRs are still unknown. To identify some of these unknown ligands and their GPCR binding...demonstrating its potential to discover new ligands for unknown GPCRs. You can find the entire PRESTO-TANGO kit...

  4. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...scanning libraries. PFunkel: Of prior existing comprehensive mutagenesis methods, PFunkel offers the best ...PMC3159719. 6. Firnberg, Elad, and Marc Ostermeier. “PFunkel: Efficient, Expansive, User-Defined Mutagenesis...

  5. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...sites (5). Imagine that you have a genome with unknown sequence. In the era before sequencing was commonplace...restriction enzyme digests. Incubating a DNA sample of unknown sequence with restriction enzymes and then running...26554401. PubMed Central PMCID: PMC4727787. 9. Hunkapiller, Tom, et al. "Large-scale and automated DNA sequence...

  6. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...being opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive when ...):443-6. doi: 10.1038/74704. PMID: 10742152. Dunkelberger, J., Song, WC. Complement and its role in innate...

  9. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...plan and break up work into small, well defined chunks, called stories, and work on stories during short...finish all of the work. Since we work in iterative chunks, we have the flexibility to adapt and easily respond...

  10. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ... can affect FRET. When it comes to studying an unknown interaction between two proteins, these parameters...difficult to address especially when you investigate unknown molecular complexes. But it is a parameter that...

  15. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ... which irreversibly excise or invert a specific chunk of DNA. However, a limitation to this strategy is... system can be used for identifying previously unknown factors and pathways involved in pre-mRNA splicing...

  16. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...knock-ins of patient mutations which are variants of unknown significance – mutations with unclear effects on...
Showing: 1 - 20 of 61 results