...on the expected concentration of the unknown sample. The unknown should fall somewhere in the middle of...determine the concentration of a target molecule in an unknown sample. To do this, you serially dilute a control...information to extrapolate the concentration of an unknown sample. For example, a BCA assay uses a serial ...dilution of a control protein, incubated alongside an unknown sample with the BCA reagent. The absorbance of ...allows you to determine the concentration of the unknown sample.  How do I make the standards for a standard... prepare the standard, standard dilutions, and unknown sample(s) in the same buffer if possible, or very..., you can extrapolate the concentration of the unknown sample based on its measured response. An example...

...Mice Unknown Kumar et al., 2020 AAV-PHP.AX CNS: neurons, astrocytes Mice Unknown Jang ... ganglion neurons Mice, rats No (mechanism unknown) Chan et al., 2017 Asencor et al., 2022 AAV-PHP.eC... Mice, rats, rhesus macaques No (mechanism unknown)   Chen et al., 2023 AAV1-X1 Brain endothelial...endothelial cells Mice No (mechanism unknown)   Chen et al., 2023 AAV9.452sub.LUNG1 Lung: ATII...CNS: neurons and astrocytes Mice, marmosets Unknown Goertsen, Flytzanis, et al., 2022 AAV.CAP-B10...inhibitory and excitatory neurons Mice, marmosets Unknown Brown et al, 2021 Goertsen, Flytzanis, et al....endothelial cells Mice, rats No (mechanism unknown) Krolak et al., 2022 AAV.MaCPNS1 PNS (mice...

...activate many GPCRs are still unknown. To identify some of these unknown ligands and their GPCR binding...demonstrating its potential to discover new ligands for unknown GPCRs. You can find the entire PRESTO-TANGO kit...

...scanning libraries. PFunkel: Of prior existing comprehensive mutagenesis methods, PFunkel offers the best ...PMC3159719. 6. Firnberg, Elad, and Marc Ostermeier. “PFunkel: Efficient, Expansive, User-Defined Mutagenesis...

...sites (5). Imagine that you have a genome with unknown sequence. In the era before sequencing was commonplace...restriction enzyme digests. Incubating a DNA sample of unknown sequence with restriction enzymes and then running...26554401. PubMed Central PMCID: PMC4727787. 9. Hunkapiller, Tom, et al. "Large-scale and automated DNA sequence...

...being opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive when ...):443-6. doi: 10.1038/74704. PMID: 10742152. Dunkelberger, J., Song, WC. Complement and its role in innate...

...these “variants of unknown significance” (sometimes called “VUS”) function is unknown. How can we make ...

...treatment can be monitored. This can reveal previously unknown functions for genes, and lays the groundwork for...research into genetic compensation, a previously unknown mechanism of gene regulation (El-Brolosy et al....

...plan and break up work into small, well defined chunks, called stories, and work on stories during short...finish all of the work. Since we work in iterative chunks, we have the flexibility to adapt and easily respond...

... can affect FRET. When it comes to studying an unknown interaction between two proteins, these parameters...difficult to address especially when you investigate unknown molecular complexes. But it is a parameter that...

... has been predicted that many others are still unknown. Identifying Cas14 variants In this quest to discover...

...genes. Using patient data to identify mutations of unknown importance in cancer Previously available methods...

...technique, and plasmids to study the expression of an unknown protein in macrophages/monocytes using lentiviral...

...bacterial toxin resistance, identifying 4 previously unknown genes.  “With this work, it is now possible to ...

...knock-ins of patient mutations which are variants of unknown significance – mutations with unclear effects on...

....180241 Vanni, C., et al., Unifying the known and unknown microbial coding sequence space. eLife. (2022) ...

... which irreversibly excise or invert a specific chunk of DNA. However, a limitation to this strategy is... system can be used for identifying previously unknown factors and pathways involved in pre-mRNA splicing...

...initial read, adjust my language in the first (and clunky) version of my general comments and adjust them...

... two weeks and with labs worldwide closing for unknown amounts of time, we were facing a financial crisis...

...conditions for culturing or long-term stabilization of unknown bacteria. Figure 2: Christina Mork collecting...