We narrowed to 34 results for: XIA
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TypeBlog PostPublishedAug. 2, 2016, 2:30 p.m....circular DNA with staggered nicks, recent data (see Xia et al., 2015) suggests that the final PCR products...Science 239 (1988): 487-491. PubMed PMID: 2448875. 4. Xia, Yongzhen, et al. "New insights into the QuikChangeTM...
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Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog PostPublishedOct. 10, 2017, 1:57 p.m....microscopy project excitation light through the entire axial volume of a sample. Therefore, when a large number... of field of the objective (the distance in the axial dimension that will appear in focus). This means...photobleaching when acquiring multiple images over a large axial range. 2-photon imaging, which restricts excitation... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m....for genome engineering applications. Image from Xiaoshu, et. al. 2021. Created by the fusion of Cas12f...possibilities in both cell engineering and gene therapy. Xiaoshu X, et al. Molecular Cell. 2021. https://doi.org... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog PostPublishedJan. 26, 2016, 3:30 p.m.... diseases caused by splicing defects, including ataxia telangiectasia, congenital disorder of glycosylation..., Chew WL, Widrick JJ, Yan WX, Maesner C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church... -
What's New in CRISPR - Spring 2019
TypeBlog PostPublishedMarch 21, 2019, 12:47 p.m.... editing with a Cas9-adenosine deaminase fusion Caixia Gao’s lab has developed a new plant base editor... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m.... A new genome-wide CRISPR knockout library from Xiaole Shirley Liu's lab targets all mouse genes with ... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....Pacouret S, Bouzelha M, Shelke R, Andres-Mateos E, Xiao R, Maurer A, Mevel M, Turunen H, Barungi T, Penaud-Budloo... -
Plasmids 101: Origin of Replication
TypeBlog PostPublishedNov. 11, 2020, 2:23 p.m....comments. References Chatterjee A, Sun SB, Furman JL, Xiao H, Schultz PG. A versatile platform for single- ... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....DNA without requiring a double-stranded break. The Xiao Wang Lab has recently deposited a set of plasmids... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....First Impressions Here References 1. Gao, Feng, Xiao Z. Shen, Feng Jiang, Yongqiang Wu & Chunyu Han. ... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog PostPublishedAug. 4, 2020, 1:15 p.m.... Li P, Mi D, Ren L, Guo L, Guo R, Chen T, Hu J, Xiang Z, Mu Z, Chen X, Chen J, Hu K, Jin Q, Wang J, Qian... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m.... plasmids from this paper at Addgene. 3. Yan, Xiaowei, et al. “Dynamics of Translation of Single mRNA... -
Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m....activity of CAR–T cells was recently devised by the Yingxiao Wang lab. The crux of the system is a set of ... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....resources References Dolan, E., Wang, Hou, Zhonggang, Xiao, Yibei, et. al. Introducing a Spectrum of Long-Range... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog PostPublishedMarch 12, 2020, 1:15 p.m....Lukáts, Á., Németh, J., Nagy, Z.Z., Wu, K., Wu, R., Xiang, L., Fang, X., Jin, Z., Goldblum, D., Hasler, P.... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m....transduction experiment. References 1. Kutner, Robert H., Xian-Yang Zhang, and Jakob Reiser. "Production, concentration... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....e3. https://doi.org/10.1016/j.molcel.2018.03.031 Xiao, Y., Luo, M., Dolan, A. E., Liao, M., & Ke, A. (... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....doi.org/10.1089/hum.2009.223 Vandenberghe, L. H., Xiao, R., Lock, M., Lin, J., Korn, M., & Wilson, J. M... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016). “Highly efficient CRISPR... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....illumination microscope (SPIM). In order to improve axial resolution and reduce phototoxicity for imaging ...