We narrowed to 45 results for: XIA
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TypeBlog Post...circular DNA with staggered nicks, recent data (see Xia et al., 2015) suggests that the final PCR products...Science 239 (1988): 487-491. PubMed PMID: 2448875. 4. Xia, Yongzhen, et al. "New insights into the QuikChangeTM...
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Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post...microscopy project excitation light through the entire axial volume of a sample. Therefore, when a large number... of field of the objective (the distance in the axial dimension that will appear in focus). This means...photobleaching when acquiring multiple images over a large axial range. 2-photon imaging, which restricts excitation... -
Hot Plasmids - February 2022
TypeBlog Post...for genome engineering applications. Image from Xiaoshu, et. al. 2021. Created by the fusion of Cas12f...possibilities in both cell engineering and gene therapy. Xiaoshu X, et al. Molecular Cell. 2021. https://doi.org... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post... diseases caused by splicing defects, including ataxia telangiectasia, congenital disorder of glycosylation..., Chew WL, Widrick JJ, Yan WX, Maesner C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church... -
Neurodegeneration Plasmid Collection
TypeCollection...Episodic ataxia Xiaolong Wang 159788 pGL3-U6-sgRNA_KCNA1-mcherry KCNA1 U6 Episodic ataxia Xiaolong Wang 159912...Spinocerebellar ataxia 30 Frederic Mushinski 8418 pLTR PKC gamma PRKCG Spinocerebellar ataxia 29 Frederic ...Friedreich ataxia Ronald Kahn 11443 pBS human Frataxin (myc tag) FXN Myc T7 Friedreich ataxia Ronald Kahn...FXN Friedreich ataxia Ronald Kahn 11504 pBabe hygro human Frataxin FXN Friedreich ataxia Ronald Kahn 11505...14542 pLV.ATMi ATM H1 Ataxia telangiectasia Didier Trono 14581 pSUPER.ATMi ATM H1 Ataxia telangiectasia Didier...Friedreich ataxia Michael Ristow 14977 pCINeo-hFX(I154F)-HA FXN HA, AU1 CMV Friedreich ataxia Michael Ristow...Friedreich ataxia Michael Ristow 14979 pGL3-basic-hFX-promotor(1,3kb) FXN hFXN Friedreich ataxia Michael ... -
What's New in CRISPR - Spring 2019
TypeBlog Post... editing with a Cas9-adenosine deaminase fusion Caixia Gao’s lab has developed a new plant base editor... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post... A new genome-wide CRISPR knockout library from Xiaole Shirley Liu's lab targets all mouse genes with ... -
Plasmids 101: Origin of Replication
TypeBlog Post...comments. References Chatterjee A, Sun SB, Furman JL, Xiao H, Schultz PG. A versatile platform for single- ... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...DNA without requiring a double-stranded break. The Xiao Wang Lab has recently deposited a set of plasmids... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...Pacouret S, Bouzelha M, Shelke R, Andres-Mateos E, Xiao R, Maurer A, Mevel M, Turunen H, Barungi T, Penaud-Budloo... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...First Impressions Here References 1. Gao, Feng, Xiao Z. Shen, Feng Jiang, Yongqiang Wu & Chunyu Han. ... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post... plasmids from this paper at Addgene. 3. Yan, Xiaowei, et al. “Dynamics of Translation of Single mRNA... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post... Li P, Mi D, Ren L, Guo L, Guo R, Chen T, Hu J, Xiang Z, Mu Z, Chen X, Chen J, Hu K, Jin Q, Wang J, Qian... -
Hot Plasmids - January 2023
TypeBlog Post...activity of CAR–T cells was recently devised by the Yingxiao Wang lab. The crux of the system is a set of ... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post...resources References Dolan, E., Wang, Hou, Zhonggang, Xiao, Yibei, et. al. Introducing a Spectrum of Long-Range... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...Lukáts, Á., Németh, J., Nagy, Z.Z., Wu, K., Wu, R., Xiang, L., Fang, X., Jin, Z., Goldblum, D., Hasler, P.... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post...transduction experiment. References 1. Kutner, Robert H., Xian-Yang Zhang, and Jakob Reiser. "Production, concentration... -
Viral Vectors 101: Producing Your rAAV
TypeBlog Post...doi.org/10.1089/hum.2009.223 Vandenberghe, L. H., Xiao, R., Lock, M., Lin, J., Korn, M., & Wilson, J. M... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...e3. https://doi.org/10.1016/j.molcel.2018.03.031 Xiao, Y., Luo, M., Dolan, A. E., Liao, M., & Ke, A. (... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016). “Highly efficient CRISPR...