...an antibiotic resistance marker from the vector (abR, Figure 1). The resolution step (“double crossover...

...in ordering from ABRC for the first time, we recommend reaching out to us at ABRCedu@osu.edu for help ...Growing capacity - ABRC outreach today Over the years, the outreach efforts of ABRC have grown. A few ...festivals, ABRC gets the word out about the importance of plant sciences. In 2017 alone, ABRC staff reached...1991, the Arabidopsis Biological Resource Center (ABRC) is one of two global stock centers for Arabidopsis... at The Ohio State University in Columbus, Ohio, ABRC ships more than 100,000 samples to researchers and...crisis. The Arabidopsis Biological Resource Center (ABRC) set out to remedy that with the establishment of...and support from the National Science Foundation, ABRC’s outreach program kicked off with the release of...

...library! Now also available as a lentiviral prep. Calabrese: human CRISPRa sgRNA library In addition to inhibiting... A and B. Sanson et al. demonstrated that the Calabrese library substantially identified more hits than...al., 2015). Root and Doench also compared the Calabrese library to screens performed with open reading...for resistance to MEK inhibition. Although both Calabrese and the ORF overexpression library identified ...in complement to study gene function. Find the Calabrese pooled library and lentiviral prep here! Applications...

... and downstream signaling cascades (reviewed in Abreu & Levitz, 2020), including light-sensitive chimeric...! Resources and references Recommended reading Abreu, N., & Levitz, J. (2020). Optogenetic Techniques.... https://doi.org/10.1038/nmeth.1808 References Abreu, N., & Levitz, J. (2020). Optogenetic Techniques...

... for a change.  Working abroad: Research in Australia I then travelled abroad to work at the University... scientists experience during research. Working abroad in rural Africa Once this position ended, I wanted...

...above 600 nm) and increasing the brightness. Arne Fabritius and colleagues started with the FP mNeptune684...and a bacterial expression vector (pRSETB). Fabritius A, et al. Cell Chemical Biology 2018. PubMed: ...

...discussions about the pros and cons of different fabrics while we worked. We also had discussions about ...aerosolised droplet transmission and how different fabrics and mask designs could work to slow the spread ...

...were first expressed in E. coli in the 1980s  (Kanabrocki et al 1986). Much later, it was shown that putting...her on Twitter @jessicapolka.     References 1. Kanabrocki, J. A., Quackenbush, R. L. & Pond, F. R. Organization...

...in vitro analysis, IDLV for in vivo detection (Gabriel et al. 2011, Wang et al. 2015, Osborn et al. 2016....3 (2015): 237-243. PubMed PMID: 25664545. 10. Gabriel, Richard, et al. "An unbiased genome-wide analysis...

...recognition sites (i.e., loxP and FRT, respectively) (Abremski and Hoess, 1984; Van Duyne, 2001; Christenson ...PMID: 29743652. PubMed Central PMCID: PMC5943452. Abremski, Ken, and Ronald Hoess. "Bacteriophage P1 site-specific...

...Flies 101: Catching flies in the lab References Abril, U.N.O., et. al. (2022) A plasmid-based system for...

...deeply concerned about these developments. These abrupt changes threaten to slow transformative biological...

...monitoring in vivo. To overcome these technical issues, Sabrina Büttner’s lab designed a calcineurin transcriptional...

...translation system to examine biosynthetic pathways  By Gabrielle Clouse Recently, cell-free transcription/translation...

... all enjoy it!Disclaimer: I had to cut it very abruptly to stay within the time limit. pic.twitter.com...

... employing synthetic biology resources and microfabrication technologies to generate a self renewable ...

...given application is a challenge. To help keep you abreast of the latest fluorescent proteins, I maintain ...

... Cas12a for multiplexed gene perturbation by: Gabrielle Clouse While many CRISPR-based genetic screening...

...everything molecular biology. Follow him on Twitter @AnotherLabRat.     References 1. Miyaoka, Y. et al. Isolation...

...Musunuru: The kit includes 832 plasmids containing prefabricated pieces that allow you to build a DNA-binding...