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  2. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

    Type
    Blog Post
    ...in ordering from ABRC for the first time, we recommend reaching out to us at ABRCedu@osu.edu for help ...Growing capacity - ABRC outreach today Over the years, the outreach efforts of ABRC have grown. A few ...festivals, ABRC gets the word out about the importance of plant sciences. In 2017 alone, ABRC staff reached...1991, the Arabidopsis Biological Resource Center (ABRC) is one of two global stock centers for Arabidopsis... at The Ohio State University in Columbus, Ohio, ABRC ships more than 100,000 samples to researchers and...crisis. The Arabidopsis Biological Resource Center (ABRC) set out to remedy that with the establishment of...and support from the National Science Foundation, ABRC’s outreach program kicked off with the release of...

  3. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...library! Now also available as a lentiviral prep. Calabrese: human CRISPRa sgRNA library In addition to inhibiting... A and B. Sanson et al. demonstrated that the Calabrese library substantially identified more hits than...al., 2015). Root and Doench also compared the Calabrese library to screens performed with open reading...for resistance to MEK inhibition. Although both Calabrese and the ORF overexpression library identified ...in complement to study gene function. Find the Calabrese pooled library and lentiviral prep here! Applications...

  4. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ... and downstream signaling cascades (reviewed in Abreu & Levitz, 2020), including light-sensitive chimeric...! Resources and references Recommended reading Abreu, N., & Levitz, J. (2020). Optogenetic Techniques.... https://doi.org/10.1038/nmeth.1808 References Abreu, N., & Levitz, J. (2020). Optogenetic Techniques...

  5. Finding Your Perfect Job After University

    Type
    Blog Post
    ... for a change.  Working abroad: Research in Australia I then travelled abroad to work at the University... scientists experience during research. Working abroad in rural Africa Once this position ended, I wanted...

  7. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...discussions about the pros and cons of different fabrics while we worked. We also had discussions about ...aerosolised droplet transmission and how different fabrics and mask designs could work to slow the spread ...

  8. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...were first expressed in E. coli in the 1980s  (Kanabrocki et al 1986). Much later, it was shown that putting...her on Twitter @jessicapolka.     References 1. Kanabrocki, J. A., Quackenbush, R. L. & Pond, F. R. Organization...

  10. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...in vitro analysis, IDLV for in vivo detection (Gabriel et al. 2011, Wang et al. 2015, Osborn et al. 2016....3 (2015): 237-243. PubMed PMID: 25664545. 10. Gabriel, Richard, et al. "An unbiased genome-wide analysis...

  11. CRISPR Library Amplification

    Type
    Protocol
    ...have a sharp edge that can scrape plates more abrasively at a certain angle. Incubate plates upside down...

  12. The Pleiades Promoter Project

    Type
    Collection
    ...intron-lacZ/NLS Ple77 GABRA6 pEMS1431 EGFP/NLS Ple78 GABRA6 pEMS1058 cre/EGFP/NLS Ple78 GABRA6 pEMS1432 EGFP/...EGFP/NLS Ple79 GABRA6 pEMS1433 EGFP/NLS Ple79 GABRA6 pEMS1550 intron-lacZ/NLS Ple80 GABRA6 pEMS1434 EGFP/...

  20. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGTTGTCAATGATGCTGTgctttattcttggctgCTCCATGGCAAACACCGA gabra4 TAL3076 & TAL3077 TAAAGAGAATATCTGGACagatcagaaaggatgaGAAATTATACCCGGAAAA gabra5 TAL 3014...TGATAACATCACAGTATTcacacgaatccttgacGGATTGCTGGATGGCTA gabrg2 TAL3078 & TAL3079 TGGAAAGCGATGATGACGaagtgacaaacaaaacATGGGTTCTGACTCCCAA...
Showing: 1 - 20 of 53 results