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  1. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...Anti-Integrin alpha L [IPI-TS2/4] Integrin alpha-L Human IgG1 Rabbit Anti-Integrin alpha L [IPI-M17/... Integrin alpha-L Mouse IgG1 Rabbit Anti-Integrin alpha L [IPI-TS1/22] Integrin alpha-L Human IgG1...Anti-Integrin alpha M [IPI-M1/70] Integrin alpha-M Mouse, Human IgG1 Rabbit Anti-Integrin alpha M [IPI-CBRM1... Integrin alpha-M Human IgG1 Rabbit Anti-Integrin alpha M [IPI-CBRM1/29] Integrin alpha-M Human IgG1...Anti-Integrin alpha M [IPI-LM2/1] Integrin alpha-M Human IgG1 Rabbit Anti-Integrin alpha 5 [IPI-MFR5...] Integrin alpha-5 Mouse IgG1 Rabbit Anti-Integrin alpha L [IPI-TS2/4] Integrin alpha-L Human IgG2a...Anti-Integrin alpha L [IPI-M17/4] Integrin alpha-L Mouse IgG2a Rat Anti-Integrin alpha M [IPI-M1/70...
  2. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...blocked by alpha2-AR antagonist, nLight sensors are not. Instead, they are blocked by alpha1-AR antagonist...., 2023. nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains circularly...antagonist. This is important because alpha2-AR can provide inhibitory feedback in response to norepinephrine...multiplexing in experiments, including ones that study alpha2-AR. Selectivity Norepinephrine and dopamine are...
  3. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro: Expression of the SARS-CoV-2 N protein  pLVX-EF1alpha-SARS-CoV...the E protein (pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro) and NSP2 (pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro...2xStrep-IRES-Puro: Expression of the SARS-CoV-2 M protein pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro: Expression...
  4. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...in cell lines expressing galactosyl(alpha1-3)galactosyl (alphaGal) sugars were less stable than viruses...express these sugars. (Takeuchi et al., 1997). The alphaGal sugars end up in the envelope and are targets ...targets for complement-based killing by anti-alphaGal antibodies. The VSV-G envelope protein is inactivated ..., and spumaviruses to human serum by galactosyl(alpha1-3)galactosylation. Journal of virology 71:6174–...
  5. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...data points fall to each other (see Fig. 1). Alpha values – numerical risk allowance in a statistical...comparisons are performed. For example, if your alpha is set at .05, then 5% of the time the statistical...threshold at a p value less than or equal to the alpha value divided by the number of null hypotheses. ...number of comparisons performed multiplied by the alpha value is less than or equal to the p value. There...
  6. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...Camelid family is composed of camels, llamas, and alpacas (and their undomesticated counterparts) - not exactly...soon after found similar antibodies in llamas and alpacas (Figure 1). At the time, researchers were already...started with immunizing a camelid (usually llamas or alpacas). But because sdAbs are so amenable to recombinant...
  7. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...transmembrane domain receptors, due to the seven alpha helices that transverse the cellular membrane. The...pathways and consist of a trimer of three subunits: alpha (Gα), beta (Gβ), and gamma (Gγ). G proteins are ...plasmid kit consists of 20 plasmids encoding 14 alpha, 2 beta, and 4 gamma subunits of the heterotrimeric...
  8. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...antibody (HCab), which are unique to camels, llamas, alpacas and other camelids. In terms of structure, HCabs...have done the hard work for you by immunizing an alpaca, isolating HCab antibodies from its serum, and ...for generating nanobodies are outlined below; no alpaca required. Decide Which Nanobody To Use: The table...
  9. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...with the transposition protein TniQ for targeting (Halpin-Healy et al., 2020). Genomic targets require an...follow him on twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg SH, Fernández IS...doi.org/10.1038/s41586-019-1849-0 Klompe SE, Vo PLH, Halpin-Healy TS, Sternberg SH (2019) Transposon-encoded...
  10. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...FRET biosensors (yellow cameleon 3.60, EPAC, and Galphai), the C-terminus and N-terminus of the donor fluorescent... in between secondary structure (beta-sheets or alpha helices). An additional requirement is that the ...tagging of Ccd42 (Bendezú et al., 2015) and the alpha subunit of heterotrimeric G-proteins (Janetopoulos...
  11. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...complex consists of a trimer of three subunits: alpha, beta, and gamma (Gα, Gβ, and Gγ), collectively ...transfer 2, or BRET2. With binding of the drug, the alpha and beta subunits dissociate resulting in decreased...
  12. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...resources References Janeway CA Jr, Travers P, Walport M, et al. Immunobiology: The Immune System in Health...www.ncbi.nlm.nih.gov/books/NBK27106/  Janeway CA Jr, Travers P, Walport M, et al. Immunobiology: The Immune System in Health...
  13. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...behavior can be monitored in an open field test (Alpar et al., 2018). Locomotion can also be used as behavioral...  future blog post, so stay tuned!  References Alpár, Alán, et al. "Hypothalamic CNTF volume transmission...
  14. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... common lab strains MG1655 and its derivatives DH5alpha and DH10b (also known as TOP10). The history of...araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ- DH5alpha   General cloning and storage of common plasmids...
  15. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ... al. modified the GCN4 sequence to increase its alpha-helical structure and stability, creating v4 SunTag...
Showing: 1 - 20 of 52 results