...use. Reagent Preparation 100 mM Valproic Acid Dissolve 2.88 g valproic acid in 200 mL deionized water ..., VWR 430770 Trypan Blue, Thermo Fisher T10282 Valproic acid sodium salt, Sigma Aldrich P4543-10G Polyethylenimine...flask to the incubator. Section 3: BCD Feed and valproic acid supplementation During the 24–144 h post-...post-transfection, supplement the flask with 3.75 mM valproic acid. Example feeding schedule: Thursday: Transfect...BCD Feed. Monday (96 h post-transfection): Add valproic acid to 3.75 mM, add 4% BCD Feed. Tuesday (120...

...NEB #M0202S T4 DNA ligase buffer NEB #B0202S DH5 alpha competent cells Invitrogen #18258-012 Qiaquick gel...Transform 2 μL of ligation mix into 25 μL competent DH5 alpha cells, following manufacturer’s protocol. Plate ...

...ligation reaction into competent cells such as DH5alpha or TOP10. If using much less total DNA (<1ng...

...ligation reaction into competent cells such as DH5alpha or TOP10. If using much less total DNA (<1ng) ...