Antibodies 101: Flow Compensation
Type
Blog Post
Published
May 16, 2024, 1:15 p.m.
...applied to APC-Cy7 against APC to correct for this bleed. A compensation value of 3.7 for APC into APC-Cy7 applied...you will need compensation between APC and APC-Cy7; PE and PE-Cy7; APC-Cy7 and PE-Cy7; or BV421 and BV51...voltages.
Figure 1A shows a single-color APC control, with APC and APC-CY7 plotted against each other. Since... out of APC-Cy7. After increasing the compensation values for APC against APC-Cy7, the populations are...only be positive for APC, yet it seems as if the beads are also positive for APC-Cy7. You therefore would...would say that APC is “bleeding,” or spilling over, into APC-Cy7 channel and needs to be compensated out... showing an APC single-color control sample before (A) and after (B) compensation with APC-Cy7. The positive...