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We narrowed to 33 results for: fau

Showing: 1 - 20 of 33 results
  1. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ... from the dropdown menu. Typically, we use the default nucleotide database “Nucleotide collection (nr/... as well as the overall alignment results. The default setting is “Optimize for Highly similar sequences...results. When using blastx at Addgene, we use the default “Non-redundant protein sequences (nr)” database...repeated or highly homologous regions, which by default are ignored in a standard blastx search. An alignment...have found it saves analysis time to remove this default setting. Timesaving Tip #2: blastx searches are...searches do not filter low complexity regions by default, meaning that you do not have to remember to adjust...adjust any blastp algorithm parameters. We use the default scoring matrix BLOSUM62, but you may want to check...
  2. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...switch between the default RFP and CFP, while Brainbow 2.1 switches from the default GFP (green) to RFP...in the same publication) are unique because by default, they are not fluorescent. Instead, they incorporate... truncated, non-fluorescent form of YFP as the default state of the transgene such that recombination ...cells that do not express Cre fluorescing with the default RFP. The Ubow construct was then used to develop...
  3. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...mOrange2 is the default state. In contrast, Brainbow-3.1 and -3.2 do not display default fluorescence due...Third, Brainbow-1 and Brainbow-2 each contained a “default” state; for example, Brainbow-1.0 expresses RFP...construct has not undergone recombination. This default state was disproportionately expressed by a majority...
  4. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...font-weight: normal; text-decoration: none","font_set":"DEFAULT"},"button_hover_color":{"color":"#0052a9","css"...169)","rgba":"rgba(0, 82, 169, 0.75)"},"override_default_style":true,"text_hover_color":{"color":"#ffffff...
  5. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...inducible promoters, which can be switched from a default OFF to an ON state, and how you might use these...repressible promoters, which can be switched from a default ON to an OFF state, as well as repressible binary...
  6. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...font-weight: normal; text-decoration: none","font_set":"DEFAULT"},"button_hover_color":{"color":"#0052a9","css"...169)","rgba":"rgba(0, 82, 169, 0.75)"},"override_default_style":true,"text_hover_color":{"color":"#ffffff...
  7. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...CRISPR Screens Using the FITS Approach Caitlin Faulds, who introduced the very first antibody deposit...
  8. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ...the website is launched, it will show my data by default. Depositors can download their CSV file from Addgene...
  9. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ... of the benefits of preprints. Some worry about faulty work being posted as preprints, assuming that peer...
  10. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...your strategy. Don’t assume it’s the audience’s fault; instead look for ways to improve how you're communicating...
  11. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ... can be to lose time and money on a potentially faulty product. Please remember that the person at the...
Showing: 1 - 20 of 33 results