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We narrowed to 34 results for: fau

Showing: 1 - 20 of 34 results
  1. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ... from the dropdown menu. Typically, we use the default nucleotide database “Nucleotide collection (nr/... as well as the overall alignment results. The default setting is “Optimize for Highly similar sequences...results. When using blastx at Addgene, we use the default “Non-redundant protein sequences (nr)” database...repeated or highly homologous regions, which by default are ignored in a standard blastx search. An alignment...have found it saves analysis time to remove this default setting. Timesaving Tip #2: blastx searches are...searches do not filter low complexity regions by default, meaning that you do not have to remember to adjust...adjust any blastp algorithm parameters. We use the default scoring matrix BLOSUM62, but you may want to check...
  2. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...switch between the default RFP and CFP, while Brainbow 2.1 switches from the default GFP (green) to RFP...in the same publication) are unique because by default, they are not fluorescent. Instead, they incorporate... truncated, non-fluorescent form of YFP as the default state of the transgene such that recombination ...cells that do not express Cre fluorescing with the default RFP. The Ubow construct was then used to develop...
  3. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...mOrange2 is the default state. In contrast, Brainbow-3.1 and -3.2 do not display default fluorescence due...Third, Brainbow-1 and Brainbow-2 each contained a “default” state; for example, Brainbow-1.0 expresses RFP...construct has not undergone recombination. This default state was disproportionately expressed by a majority...
  4. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...font-weight: normal; text-decoration: none","font_set":"DEFAULT"},"button_hover_color":{"color":"#0052a9","css"...169)","rgba":"rgba(0, 82, 169, 0.75)"},"override_default_style":true,"text_hover_color":{"color":"#ffffff...
  5. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...inducible promoters, which can be switched from a default OFF to an ON state, and how you might use these...repressible promoters, which can be switched from a default ON to an OFF state, as well as repressible binary...
  6. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...font-weight: normal; text-decoration: none","font_set":"DEFAULT"},"button_hover_color":{"color":"#0052a9","css"...169)","rgba":"rgba(0, 82, 169, 0.75)"},"override_default_style":true,"text_hover_color":{"color":"#ffffff...
  7. CRISPR Library Amplification

    Type
    Protocol
    ...1652100) Reagents 200 µL electrocompetent cells (Default: 4 tubes of Endura Duos, Lucigen, 60242-1) Alternatives...
  8. Summer SciComm: Preprints

    Type
    Blog Post
    ... of the benefits of preprints. Some worry about faulty work being posted as preprints, assuming that peer...
Showing: 1 - 20 of 34 results