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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 5 results for: fkbp

Showing: 1 - 5 of 5 results

  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... that contain rapamycin-binding domains, such as FKBP and FRB. The cell does not appear to mind extraneous...co-expressing its replacement recombinant protein with a FKBP domain and a mitochondrial outer membrane protein...). Rapamycin binds your recombinant protein (via FKBP) and then sequesters the protein at the mitochondria...

  3. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...binding protein 12 (Cas9(C)-FKBP-2xNLS) and the n-terminal cas9 fragment with FKBP rapamycin binding domain...with rapamycin induces Cas9(N)-FRB-NES and Cas9(C)-FKBP-2xNLS dimerization and net influx into the nucleus...genes using dCas9-VP64 activator fragments (dCas9(C)-FKBP-2xNLS-VP64 and dCas9(N)-FRB-NES). This system provides...

  4. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...selective small molecule FKBP12F36V degraders (dTAG molecule) and expression of FKBP12F36V-tagged proteins. The... C-terminally FKBP12F36V-tagged fusion chimeras or locus-specific knock-in of FKBP12F36V. Detailed protocols...induces ternary complex formation between the FKBP12F36V-fusion and the ubiquitin proteasome machinery...conjugating a CRBN-binding ligand (thalidomide) to an FKBP12F36V binding ligand (ortho-AP1867) via various chemical... determine which terminus can accommodate the FKBP12F36V tag without disrupting protein function. A literature...cells expressing a control -- for example, an FKBP12F36V-tagged LACZ) with dTAG molecules in your biological...the doses used to evaluate degradation of the FKBP12F36V-fusion chimera of interest. In the cell lines...

  5. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...power of a novel degrader of the 12 kDa protein FKBP12F36V. By fusing FKPB12F36V to your protein of interest...Fig 1. dTag molecules induce dimerization of an FKBP12F36V fused target protein-of-interest and the CRBN...
Showing: 1 - 5 of 5 results