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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 7 results for: fkbp

Showing: 1 - 7 of 7 results

  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... that contain rapamycin-binding domains, such as FKBP and FRB. The cell does not appear to mind extraneous...co-expressing its replacement recombinant protein with a FKBP domain and a mitochondrial outer membrane protein...). Rapamycin binds your recombinant protein (via FKBP) and then sequesters the protein at the mitochondria...

  3. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...binding protein 12 (Cas9(C)-FKBP-2xNLS) and the n-terminal cas9 fragment with FKBP rapamycin binding domain...with rapamycin induces Cas9(N)-FRB-NES and Cas9(C)-FKBP-2xNLS dimerization and net influx into the nucleus...genes using dCas9-VP64 activator fragments (dCas9(C)-FKBP-2xNLS-VP64 and dCas9(N)-FRB-NES). This system provides...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...luminescence. Find plasmids with LgBit and SmBit . Cluc-FKBP and FRB-Nluc : Split firefly luciferase reporter...

  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ..., FTD Nikolai Sluchanko 100752 HIP1R-GFP-FKBP HIP1R GFP, FKBP CMV Parkinson's Stephen Royle 101223 pHR-FUSN-mCh-Cry2WT...Parkinson's Mark Cookson 193584 3xFLAG-FKBP-LRRK2 LRRK2 Flag, FKBP CMV Parkinson's Mark Cookson 193772 pENTR221...telangiectasia Gaorav Gupta 208867 pHAGE-FKBP-GFP-OPTN (2-119) OPTN GFP, FKBP CMV ALS Sascha Martens 208875 pFastBac-Dual...

  6. dTAG - You're it!

    Type
    Blog Post
    ...selective small molecule FKBP12F36V degraders (dTAG molecule) and expression of FKBP12F36V-tagged proteins. The... C-terminally FKBP12F36V-tagged fusion chimeras or locus-specific knock-in of FKBP12F36V. Detailed protocols...induces ternary complex formation between the FKBP12F36V-fusion and the ubiquitin proteasome machinery...conjugating a CRBN-binding ligand (thalidomide) to an FKBP12F36V binding ligand (ortho-AP1867) via various chemical... determine which terminus can accommodate the FKBP12F36V tag without disrupting protein function. A literature...cells expressing a control -- for example, an FKBP12F36V-tagged LACZ) with dTAG molecules in your biological...the doses used to evaluate degradation of the FKBP12F36V-fusion chimera of interest. In the cell lines...

  7. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...power of a novel degrader of the 12 kDa protein FKBP12F36V. By fusing FKPB12F36V to your protein of interest...Fig 1. dTag molecules induce dimerization of an FKBP12F36V fused target protein-of-interest and the CRBN...
Showing: 1 - 7 of 7 results