... that contain rapamycin-binding domains, such as FKBP and FRB. The cell does not appear to mind extraneous...co-expressing its replacement recombinant protein with a FKBP domain and a mitochondrial outer membrane protein...). Rapamycin binds your recombinant protein (via FKBP) and then sequesters the protein at the mitochondria...

...approaches to provide temporal control, this new system, Fkbp/Frb Inducible Recruitment for Epigenome editing ...

...binding protein 12 (Cas9(C)-FKBP-2xNLS) and the n-terminal cas9 fragment with FKBP rapamycin binding domain...with rapamycin induces Cas9(N)-FRB-NES and Cas9(C)-FKBP-2xNLS dimerization and net influx into the nucleus...genes using dCas9-VP64 activator fragments (dCas9(C)-FKBP-2xNLS-VP64 and dCas9(N)-FRB-NES). This system provides...

...Interaction Assays Cluc-FKBP , FRB-Nluc : Split Firefly luciferase to assess FKBP and FRP interactions in...

..., FTD Nikolai Sluchanko 100752 HIP1R-GFP-FKBP HIP1R GFP, FKBP CMV Parkinson's Stephen Royle 101223 pHR-FUSN-mCh-Cry2WT...Parkinson's Mark Cookson 193584 3xFLAG-FKBP-LRRK2 LRRK2 Flag, FKBP CMV Parkinson's Mark Cookson 193772 pENTR221...telangiectasia Gaorav Gupta 208867 pHAGE-FKBP-GFP-OPTN (2-119) OPTN GFP, FKBP CMV ALS Sascha Martens 208875 pFastBac-Dual...

...selective small molecule FKBP12F36V degraders (dTAG molecule) and expression of FKBP12F36V-tagged proteins. The... C-terminally FKBP12F36V-tagged fusion chimeras or locus-specific knock-in of FKBP12F36V. Detailed protocols...induces ternary complex formation between the FKBP12F36V-fusion and the ubiquitin proteasome machinery...conjugating a CRBN-binding ligand (thalidomide) to an FKBP12F36V binding ligand (ortho-AP1867) via various chemical... determine which terminus can accommodate the FKBP12F36V tag without disrupting protein function. A literature...cells expressing a control -- for example, an FKBP12F36V-tagged LACZ) with dTAG molecules in your biological...the doses used to evaluate degradation of the FKBP12F36V-fusion chimera of interest. In the cell lines...

...power of a novel degrader of the 12 kDa protein FKBP12F36V. By fusing FKPB12F36V to your protein of interest...Fig 1. dTag molecules induce dimerization of an FKBP12F36V fused target protein-of-interest and the CRBN...