Plasmids 101: Mammalian Vectors
Type
Blog Post
Published
March 25, 2014, 3:15 p.m.
...with G418 (aka Geneticin).
Neomycin should not be used for mammalian expression – instead use G418. This...Inhibits termination step of translation
2-10 ug/mL
G418/Geneticin
neo
HeLa, NIH3T3, CHO, 293HEK, Jurkat ... can be confusing since the neo/kan gene confers G418 resistance; however, like gentamicin, neomycin is...