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We narrowed to 5 results for: g418

Showing: 1 - 5 of 5 results
  1. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...with G418 (aka Geneticin). Neomycin should not be used for mammalian expression – instead use G418. This...Inhibits termination step of translation 2-10 ug/mL G418/Geneticin neo HeLa, NIH3T3, CHO, 293HEK, Jurkat ... can be confusing since the neo/kan gene confers G418 resistance; however, like gentamicin, neomycin is...
  2. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...interest to cells with efficiencies of 1-40% after G418 selection (Kohli et al 2004, Kim et al 2008). While...helpers, and used to infect recipient cells. After G418 selection, individual gene edited clones are identified...routinely generates knockin efficiencies of 1-40% with G418-selected clones. However, there are important disadvantages...For this reason, the AAV-based approach requires G418 selection of infected clones, making it unsuitable...
  3. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...resistance to several aminoglycosides, including G418 which is commonly used for eukaryotic antibiotic... cost-effective, confers mammalian resistance to G418. Con: Slow transformation   Spectinomycin/streptomycin... Kanamycin Excellent No Bacteria and eukaryotes (G418) {% icon icon_set="fontawesome-5.14.0" name="Check...
  4. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Representative Empty Backbones Neomycin (G418) Mammalian, Varies Tet-pLKO-neo - Tet-inducible ...expression Find yeast transformation plasmids with G418 selection Find nematode transgenesis plasmids with...
  5. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...kanamycin targets the prokaryotic ribosome, while G418 (geneticin) is effective at inhibiting the eukaryotic...
Showing: 1 - 5 of 5 results