28 Hot Plasmid Technologies from 2015
Type
Blog Post
Published
Dec. 23, 2015, 3:30 p.m.
...fused to an sgRNA used to direct Cas9 to a specific genomic locus. When fused to the sgRNA and co-expressed...dCas9 – the dCas9, sgRNA/RNA cargo complex localizes to the sequence targeted by the sgRNA. This allows scientists...machinery that brings the sgRNA/cargo RNA to the appropriate DNA locus. The sgRNA/RNA fusions retained their...construct. The sgRNA targeting Gaussia luciferase can be easily replaced with an sgRNA targeting your ...specific recruitment of multiple copies of VP64 to the sgRNA-targeted gene, resulting in increased activation...SV40, SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive ...and shRNAs with a reporter gene, or (4) multiple sgRNAs with Cas9 for genome engineering. The versatility...