Progress Towards a PAM-Free CRISPR
Type
Blog Post
Published
March 7, 2024, 2:39 p.m.
...CRISPR are typically selected by the ‘quality’ of the gRNA sequence, including propensity for off-targets, ...Protospacer Adjacent Motif (PAM) sequence. If the gRNA sequence is suitable, but there is no PAM next to... to the desired cut site, then a further-away gRNA where there is an available PAM must be selected.
When...While it is possible to "get lucky" and find a great gRNA with a proximal PAM right where you need it, the...reduce the PAM requirement to allow flexibility in gRNA selection and subsequent editing.
Identifying PAM-free...lesser extent NYN. This increases the flexibility of gRNA placement significantly over SpCas9 and more so ...