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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 21 results for: halo

Showing: 1 - 20 of 21 results

  1. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...the Halo-tagged protein in question, it is targeted to the proteasome for degradation. The HaloTag is ...visualization of proteins of interest. A fluorescent Halo ligand can be added to media, buffer, etc., which...Nabet et al., Chemical Biology, 2018.    HaloTag The HaloTag was initially developed as an affinity tag...straightforward system – HaloTag and its PROTAC are all that is needed to facilitate degradation. HaloTag is also the...proteolysis-targeting chimera (PROTAC) was developed for HaloTag. PROTACs are engineered molecules which target ...involves imaging at some stage, then choosing a HaloTag could pay dividends down the road. It is difficult...

  3. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP) tags, a selectable marker (floxed Cbr-unc...small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The Foerstemann lab developed a polymerase...

  4. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...) Labeling scheme. b) COS-7 cell ER labeled with Halo-Sec61β with SiR-CA (magenta) and outer mitochondrial...known as HaloTag is actually HaloTag7, and recent specialized variants run up to HaloTag11. HaloTag is now... (Gallagher et al., 2009). HaloTag: Scientists at Promega developed HaloTag in 2008 (Los et al., 2008)...2008), based on a bacterial haloalkane dehalogenase that binds a synthetic chloralkane (CA) ligand. The current...159 Trimpethoprim  (TMP-tag) Few Easy HaloTag 295 Chloralkane (CA) Many Easy CLIP-tag...proteins (often up to 10-fold more). So, labeling a HaloTag or SNAP-tag construct can help you better detect...protein of interest. For example, the Cohen lab used HaloTag for their “ticker tape” method for intracellular...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... (including His, S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA and SUMO) for optimizing expression, ...identified a family of GFP proteins in the cephalochordate Branchiostoma floridae, named bfloGFPs. They...

  6. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...bacteriorhodopsin; a comparative study of strains of Halobacterium halobium differing in pigmentation." Biochemical...Rhodopsin-like protein from the purple membrane of Halobacterium halobium." Nature new biology 233.39 (1971): 149...opsin genes, and include bacteriorhodopsins, halorhodopsins, and channelrhodopsins, the first two found... excitation, whereas yellow light activates halorhodopsins to silence neuronal activity (Figure 1) (Wiegert...of the DA neurons produce behavioral and electroencephalography evidences of arousal in mice previously...

  7. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...externally-timed bands of color with HaloTag-iPAK4 by applying different HaloTag dyes and showed they could record.... B) HEK cell with an iPAK4 fiber labeled with HaloTag-iPAK4. Scale bar 10 µm. C) Images of iPAK4 fibers...fibers in cultured rat hippocampal neurons. HaloTag dyes are switched from yellow to red at 0 h and back...

  8. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...interchangeable labeling using the HaloTag® system, the new Janelia Fluor HaloTag Ligands expand the self-labeling... self-labeling proteins would be SNAP-tag® and HaloTag®. These consist of a genetically encoded enzyme...2017). These dyes were adapted into ligands for HaloTag® and are currently offered at Promega. Due to the...

  9. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...SNAP-tag, and HaloTag fusions, while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. ...

  11. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined that an A122D mutation...changing the dye that becomes conjugated with its HaloTag domain.  Find Voltron2 plasmids here! Abdelfattah...

  14. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ...Depositor  Notes pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag AAV5 Biosensor Tim Brown New viral prep ...

  17. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes...
Showing: 1 - 20 of 21 results