Plasmids 101: Degron Tags
Type
Blog Post
Published
March 14, 2024, 1:15 p.m.
...the Halo-tagged protein in question, it is targeted to the proteasome for degradation.
The HaloTag is ...visualization of proteins of interest. A fluorescent Halo ligand can be added to media, buffer, etc., which...Adapted from Nabet et al., (2018).
HaloTag
The HaloTag was initially developed by Promega as an ...straightforward system — HaloTag and its PROTAC are all that is needed to facilitate degradation. HaloTag is also the...proteolysis-targeting chimera (PROTAC) was developed for HaloTag. PROTACs are engineered molecules which target ...involves imaging at some stage, then choosing a HaloTag could pay dividends down the road.
It is difficult...