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Nb-gp41-Halo (MoonTag-Nb-Halo)

Plasmid

#128603

Purpose

Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

Nanobody_gp41

Use

Lentiviral

Tags

GB1 and Halo

Promoter

SFFV

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_Lifeact-HaloTag7

Plasmid

#187076

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 on actin filaments

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_Lifeact-HaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_Cox8-HaloTag7

Plasmid

#187077

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in the mitochondria matrix

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_Cox8-HaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_LamP1- HaloTag7

Plasmid

#187078

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in lysosomes

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_LamP1- HaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_CalR- HaloTag7-KDEL

Plasmid

#187079

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in endoplasmic reticulum

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_CalR- HaloTag7-KDEL

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

Plasmid

#175525

Purpose

Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag10 and Tomm20-HaloTag9

Expression

Mammalian

Mutation

HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

Plasmid

#175547

Purpose

Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag7 and Tomm20-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

Plasmid

#175523

Purpose

Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag11 and Tomm20-HaloTag9

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

Plasmid

#175526

Purpose

Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag11 and Tomm20-HaloTag7

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy-

Plasmid

#169338

Purpose

Expression of the LT-Fucci(CA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(CA) (GMNN, CDT1 Human)

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-110)Cy…

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

Plasmid

#169336

Purpose

Expression of the LT-Fucci(SA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(SA) (GMNN, CDT1 Human)

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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VPS13C^Halo

Plasmid

#232864

Purpose

Expresses human VPS13C^Halo in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

VPS13C (VPS13C Human)

Tags

internal Halo

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pH6HTC_ENO1-Halo

Plasmid

#175330

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

ENO1 (ENO1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_ABCE1-Halo

Plasmid

#175335

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

ABCE1 (ABCE1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-KDEL

Plasmid

#124316

Purpose

Expresses ER specific HaloTag protein

Depositor

Jin Wang

Article

Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

Insert

Halo tag

Tags

KDEL Seq

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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TUBB5-Halo

Plasmid

#64691

Purpose

Expresses beta-tubulin labeled with Halo-tag

Depositor

Yasushi Okada

Article

Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

Insert

TUBB

Tags

Halo-tag

Expression

Mammalian

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pH6HTC_CCT2-Halo

Plasmid

#175337

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCT2 (CCT2 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PRMT1-Halo

Plasmid

#175334

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PRMT1 (PRMT1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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LAMP1-Halo

Plasmid

#221655

Purpose

Lysosomal marker LAMP1 tagged C-terminally with Halo tag

Depositor

Erika Holzbaur

Article

Basak et al Nat Commun. 2025 Aug 9;16(1):7367. doi:

Insert

LAMP1 (Lamp1 Rat)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-mitoTag

Plasmid

#240424

Purpose

Visualization of mitochondria

Depositor

Anna Akhmanova

Article

Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

Insert

actA

Tags

Halotag

Expression

Mammalian

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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