...to create BioID2, a smaller and improved promiscuous biotin ligase (Kim et al 2016). BioID2 consists of...tools are also currently available at Addgene.  BioID2: A smaller improved promiscuous biotin ligase The...for promiscuous biotinylation. Compared to BioID, BioID2 exhibits enhanced proximal labelling, requires ...proteins to specific locations in the cell.   Find BioID2 plasmids here APEX2: A more sensitive ascorbate...biotin ligases (Branon et al., 2018). BioID and BioID2 can take hours to label proteins and APEX2, although...

... has deposited HA tagged BioID2 for N-terminal fusions and Myc tagged BioID2 for C-terminal fusions. ...Lab’s new and improved version of BioID, called BioID2, is significantly smaller, allows for more selective...enhanced labeling of proximate proteins. Overall BioID2 improves the efficiency of screening for protein–protein...

...was  compared head-to-head with the E. coli and BioID2 BirA*s in the context of RaPID for their ability...when compared to a scrambled RNA motif control). BioID2 had no detectable signal at the same time point...

... advances 3.7 (2017): e1700782. PubMed PMID: PMID28776036. PubMed Central PMCID: PMC5517107. Additional...

...Mathews and Levin, 2018) available at Addgene: MerMAID2 - A metagenomically discovered marine anion-conducting...