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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22 results for: igl

Showing: 1 - 20 of 22 results

  1. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...blue (SF-Azurite-iGluSnFR), green (SF-iGluSnFR), and yellow (SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate...updates to iGluSnFR and SF-iGluSnFR have made it clear that it’s time to update our iGluSnFR post! Here...mice. Second Generation iGluSnFR: SF-iGluSnFR In the second generation of iGluSnFR, the Looger lab swapped...emission spectra of SF-Venus-iGluSnFR.A184V, iGluSnFR3 v82, and iGluSnFR3 v857. Image adapted from Aggarwal... original 2018 blog post. The origins of iGluSnFR   iGluSnFR was developed in 2013 by Loren Looger’s lab... generation of the SF-Venus-iGluSnFR (itself an improvement over iGluSnFR), Kaspar Podgorski’s lab have...is available here. Upgrades to iGluSnFR3 To improve upon the SF-iGluSnFR, the researchers did the following...

  2. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...axonal boutons of neurons expressing iGluSnFR3, iGluSnFR4f, and iGluSnFR4s. C) Spike-triggered averages for... library of iGluSnFR3 mutants, identifying the two  best-performing variants as iGluSnFR4f (fast deactivation...-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by the Loren Looger and... Kaspar Podgorski Labs now has a new version: iGluSnFR4, developed by the GENIE Project team at HHMI's...deactivation) and iGluSnFR4s (slow deactivation). Both have significantly improved brightness, kinetics, ..., and sensitivity. iGluSnFR4f is well-suited for imaging rapid synaptic events such as in the somatosensory...somatosensory cortex, while iGluSnFR4s produces larger signal-to-noise ratios in two-photon imaging, making ...

  3. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ... New glutamate indicator iGluSnFR3 Improving on the glutamate sensor iGluSnFR from Loren Looger’s lab,...compared with earlier SF-iGluSnFR. Image from Aggarwal 2022.   Find iGluSnFR3 plasmids here! Find viral...Kaspar Podgorski and colleagues have now released iGluSnFR3 (Aggarwal et al. 2022). This new version has higher...from the authors in this blog post. Figure 2: iGluSnFR3 displays enhanced brightness, expression, and ...viral prep here: pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt    Aggarwal A, et al. Glutamate indicators...In addition to the viral preps noted above for iGluSnFR3 and JEDI-2P, several biosensors already in our...

  4. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses one of the six panel discussions...Many thanks to our guest blogger David T. Riglar! David T Riglar is a postdoc at Harvard Medical School....science and public policy. Follow David on Twitter @driglar.     References 1. Alberts, Bruce, et al. "Rescuing...

  6. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...Many thanks to our guest blogger, David T. Riglar. David T Riglar is a postdoc at Harvard Medical School....post was contributed by guest blogger, David T. Riglar Advocating for Science Symposium and Workshop 2016...science and public policy. Follow David on Twitter @driglar.   References: 1. Alberts, Bruce, et al. "Rescuing...

  7. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...AAVrg pAAV-hSyn-GRAB_NE1m Glutamate sensors iGluSnFR is a useful tool from Loren Looger’s lab at Janelia...neuroscientists to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate...Serotype Name 106174  AAVrg pAAV.hSynapsin.SF-iGluSnFR.A184S Viral vectors coming soon! These vectors...

  8. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...sharing the data on the mAb3D atlas website.  The iGluSnFR3 Glutamate Sensor: Guest blogger Abhi Aggarwal ...reader up-to-date on the latest version of the iGluSnFR glutamate sensorRUBY-Red Siliques: Guest blogger...

  9. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ...non-specific cation channel ChR2 and the calcium channel LiGluR both cause ion movement that results in cell depolarization...spontaneous swimming. To do so, the lab expressed the LiGluR opsin in different subsets of spinal cord neurons...

  11. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...organizers wanted to touch on in organizer David Riglar’s blog post. Essentially the goal was to determine... Found Here Resources at Addgene  Read David Riglar's FOR Boston 2015 Blog Post Read Our Career Blog...

  12. New Viral Vectors - March 2024

    Type
    Blog Post
    Published
    March 5, 2024, 2:15 p.m.
    ...Biosensor Tian New viral vector pAAV.hSynapsin.SF-iGluSnFR.A184S AAV9 Biosensor Looger New serotype pAAV-CAG-DIO-NLS-mRuby3...

  19. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ... (H2O2 sensor), ArcLight (voltage sensor), and iGluSnFr (glutamate sensor). More examples of these biosensors...

  20. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari...
Showing: 1 - 20 of 22 results