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We narrowed to 31 results for: igl

Showing: 1 - 20 of 31 results

  1. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...blue (SF-Azurite-iGluSnFR), green (SF-iGluSnFR), and yellow (SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate...updates to iGluSnFR and SF-iGluSnFR have made it clear that it’s time to update our iGluSnFR post! Here...mice. Second Generation iGluSnFR: SF-iGluSnFR In the second generation of iGluSnFR, the Looger lab swapped...emission spectra of SF-Venus-iGluSnFR.A184V, iGluSnFR3 v82, and iGluSnFR3 v857. Image adapted from Aggarwal... original 2018 blog post. The origins of iGluSnFR   iGluSnFR was developed in 2013 by Loren Looger’s lab... generation of the SF-Venus-iGluSnFR (itself an improvement over iGluSnFR), Kaspar Podgorski’s lab have...is available here. Upgrades to iGluSnFR3 To improve upon the SF-iGluSnFR, the researchers did the following...

  2. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...axonal boutons of neurons expressing iGluSnFR3, iGluSnFR4f, and iGluSnFR4s. C) Spike-triggered averages for... library of iGluSnFR3 mutants, identifying the two  best-performing variants as iGluSnFR4f (fast deactivation...-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by the Loren Looger and... Kaspar Podgorski Labs now has a new version: iGluSnFR4, developed by the GENIE Project team at HHMI's...deactivation) and iGluSnFR4s (slow deactivation). Both have significantly improved brightness, kinetics, ..., and sensitivity. iGluSnFR4f is well-suited for imaging rapid synaptic events such as in the somatosensory...somatosensory cortex, while iGluSnFR4s produces larger signal-to-noise ratios in two-photon imaging, making ...

  3. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ... New glutamate indicator iGluSnFR3 Improving on the glutamate sensor iGluSnFR from Loren Looger’s lab,...compared with earlier SF-iGluSnFR. Image from Aggarwal 2022.   Find iGluSnFR3 plasmids here! Find viral...Kaspar Podgorski and colleagues have now released iGluSnFR3 (Aggarwal et al. 2022). This new version has higher...from the authors in this blog post. Figure 2: iGluSnFR3 displays enhanced brightness, expression, and ...viral prep here: pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt    Aggarwal A, et al. Glutamate indicators...In addition to the viral preps noted above for iGluSnFR3 and JEDI-2P, several biosensors already in our...

  4. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses one of the six panel discussions...Many thanks to our guest blogger David T. Riglar! David T Riglar is a postdoc at Harvard Medical School....science and public policy. Follow David on Twitter @driglar.     References 1. Alberts, Bruce, et al. "Rescuing...

  6. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...Many thanks to our guest blogger, David T. Riglar. David T Riglar is a postdoc at Harvard Medical School....post was contributed by guest blogger, David T. Riglar Advocating for Science Symposium and Workshop 2016...science and public policy. Follow David on Twitter @driglar.   References: 1. Alberts, Bruce, et al. "Rescuing...

  7. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...AAVrg pAAV-hSyn-GRAB_NE1m Glutamate sensors iGluSnFR is a useful tool from Loren Looger’s lab at Janelia...neuroscientists to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate...Serotype Name 106174  AAVrg pAAV.hSynapsin.SF-iGluSnFR.A184S Viral vectors coming soon! These vectors...

  8. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...sharing the data on the mAb3D atlas website.  The iGluSnFR3 Glutamate Sensor: Guest blogger Abhi Aggarwal ...reader up-to-date on the latest version of the iGluSnFR glutamate sensorRUBY-Red Siliques: Guest blogger...

  11. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...organizers wanted to touch on in organizer David Riglar’s blog post. Essentially the goal was to determine... Found Here Resources at Addgene  Read David Riglar's FOR Boston 2015 Blog Post Read Our Career Blog...

  12. Biosensor AAV Preps

    Type
    Collection
    ...iGABASnFR2 Glutamate Sensors iGluSnFr SF-iGluSnFr Glucose Sensors iGlucoSnFr Norepinephrine (NE) Sensors...GENIE Glutamate Sensor: iGluSnFr 98929 pAAV.hSyn.iGluSnFr.WPRE.SV40 Syn iGluSnFr none Constitutive 1, 5...Sensor: SF-iGluSnFr 106174 pAAV.hSynapsin.SF-iGluSnFR.A184S Syn SF-iGluSnFR.A184S none Constitutive 1, 9...pAAV.GFAP.SF-iGluSnFR.A184S GFAP SF-iGluSnFR.A184S none Constitutive 1 Looger Glucose Sensor: iGlucoSnFr 164510... 5, 9 Looger 98930 pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 GFAP iGluSnFr none Constitutive 1, 5, 9 Looger 98931...98931 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Syn iGluSnFr none Cre dependent 1, 5, 9 Looger 98932 pAAV.CAG.Flex.iGluSnFr.WPRE.SV40...Constitutive 5 Khakh 175180 pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt Syn iGluSnFr3 v857 none Cre dependent...

  13. Immunology Research Plasmids and Resources

    Type
    Collection
    ...-41 (non-functional) A14 IGL@ immunoglobulin lambda locus IGL, MGC88804 IGLC1 immunoglobulin lambda constant...marker) IGLC IGLC2 immunoglobulin lambda constant 2 (Kern-Oz- marker) IGLC, MGC20392, MGC45681 IGLC3 immunoglobulin...marker) IGLC IGLC6 immunoglobulin lambda constant 6 (Kern+Oz- marker, gene/pseudogene) IGLC IGLC7 immunoglobulin... C7 IGLJ@ immunoglobulin lambda joining group IGLJ IGLJ1 immunoglobulin lambda joining 1 J1 IGLJ2 immunoglobulin...6 - IGLJ7 immunoglobulin lambda joining 7 J7 IGLV@ immunoglobulin lambda variable group IGLV IGLV1-36 ...variable 1-36 IGLV136, V1-11 IGLV1-40 immunoglobulin lambda variable 1-40 IGLV140, V1-13 IGLV1-44 immunoglobulin...variable 1-44 IGLV144, V1-16 IGLV1-47 immunoglobulin lambda variable 1-47 IGLV147, V1-17 IGLV1-50 immunoglobulin...

  14. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ..., Tobias Rose AV-1-PV2723 98929-AAV1 pAAV.hSyn.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-1-PV2724 98931... 98931-AAV1 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-1-PV2725 98932-AAV1 pAAV.CAG.Flex.iGluSnFr.WPRE.SV40...Douglas Kim AV-1-PV2914 98930-AAV1 pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-1-PV3080... Baljit Khakh AV-5-PV2723 98929-AAV5 pAAV.hSyn.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-5-PV2724 98931... 98931-AAV5 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-5-PV2725 98932-AAV5 pAAV.CAG.Flex.iGluSnFr.WPRE.SV40...Douglas Kim AV-5-PV2914 98930-AAV5 pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-5-PV3107...Eric Schreiter AV-9-PV2723 98929-AAV9 pAAV.hSyn.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-9-PV2724 98931...

  15. New Viral Vectors - March 2024

    Type
    Blog Post
    ...Biosensor Tian New viral vector pAAV.hSynapsin.SF-iGluSnFR.A184S AAV9 Biosensor Looger New serotype pAAV-CAG-DIO-NLS-mRuby3...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...monitor glucose levels and transport (FLIIglu) Frommer Lab FLIIglu Plasmids Wolf Frommer Glucose Green ...S2451-9456(21)00301-9. Takashi Tsuboi Glucose iGlucoSnFR-TS biosensor for intracellular glucose Quantitative...Neurosci Res. 2019 May 20. Gary Yellen Glucose iGlucoSnFR biosensors for cytosolic glucose Looger Lab Glucose...Glutamate Fourth generation glutamate sensors (iGluSnFR4) for improved sensitivity and scale (4s) or speed...Glutamate Third generation glutamate sensors (iGluSnFR3) for improved imaging of synaptic transmission...Frommer Glutamate Ultrafast glutamate sensors (iGluSnFR variants) Ultrafast glutamate sensors resolve ...displayed variants of fluorescent glutatmate sensors (iGluSnFR1) Genetically Encoded Glutamate Indicators with...
Showing: 1 - 20 of 31 results