We narrowed to 12 results for: lacZ

...based on the ability to correctly assemble a lacI/lacZ cassette (designed by NEB for use in optimization...coding sequence for beta-galactosidase in the lacI/lacZ cassette. Additional confirmation of accurate assemblies...showed the expected complete sequence for the lacI/lacZ genes (1), while sequencing of white colonies showed...blue phenotype. Indeed, this was seen in all lacI/ lacZ assembly test systems; no blue colonies were obtained...from 1-, 12- and 24-fragment assemblies of the lacI/lacZ cassette, and illustrates how the volume of the ...fidelity and assembly efficiency Five fragment lacI/lacZ cassette assembly was easily achievable with high...both the 12- and 24-fragment versions of the lacI/lacZ cassette were designed and synthesized. In conjunction...

...the blue-white screen, which relies on the lacZ gene. lacZ encodes the enzyme 𝛽-galactosidase which can...screening at Addgene! Some bacterial strains contain lacZ in their genome, and scientists have found that ...gene of interest is inserted into the middle of the lacZ gene, thus disrupting 𝛽-galactosidase activity....

...overproduced, expression from pLac repressed more   LacZ β-galactosidase activity abolished   lacY Lactose... the potential to recombine.  F' proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR) ∆(ara-leu) 7697 araD139 fhuA...recA1 relA1 lac glnV44 F'[ ::Tn10 proAB+ lacIq Δ(lacZ)M15] hsdR17(rK- mK+)  XL10 Gold Tetracycline and...

...well-characterized bacterial lac operon contains a gene called lacZ that encodes for the enzyme β-galactosidase. Expression...Scientists discovered that deleting a section from the lacZ gene (a mutation called lacZΔM15) creates a non-...

...involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. pPD80_08) and following the expression...

...LacZ𝛼 complexes with LacZ𝜔 and forms an active LacZ tetramer.  This tetramer cleaves chlorophenol red-b-D-galactopyranoside...

...expressing a control -- for example, an FKBP12F36V-tagged LACZ) with dTAG molecules in your biological assay of...

...toxin-antitoxin system, they were able to monitor lacZ activity over 48 hours in vitro without antibiotic...

...yeast Gal4 activator, bacterial LexA DBD and the lacZ reporter gene (3, 4), there are now many variations...

...genome; does not restrict methylated DNA fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10...

...long recombinogenic homology sequence to deliver LacZ to muscle in mice. Expression from the hybrid vector...

...Cas9, EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2 can be combined with custom-cloned...