...developed photoconvertible FPs like PsMOrange and mEos add another layer of complexity to imaging, as they...encountered by some investigators when using forms of mEos." The ability to repurpose GFP is great news for...

...photoconvertible proteins are PA-GFP, Dendra2, and the mEOS proteins. Some proteins are reversibly switchable...

... Tandem UV-Violet Green/ Red N/A High 569/ 581 mEos Monomer UV-Violet Green/ Red N/A High 573/ 584 ... conditions- mEos4a and mEos4b (12). Both variants are more monomeric than mEos2 (mEos4b is completely.... green to red, or cyan to green). For example, mEosFP (named after the goddess of dawn in Greek mythology...mythology) switches from green to red fluorescence. mEosFP exposure to UV light causes an irreversible cleavage... over the monomeric one since the formation of mEosFP’s chromophore requires a temperature below 30°C ...Dendra2 is another monomeric PA-FP which unlike mEosFP, can form it’s chromophore at a more comfortable...

... you see someone who gives a great talk, ask them for advice on speaking. If you see someone with a great...circle. Asking someone to be your mentor Mentoring is a “loaded” word.  If you ask someone to be your mentor...some of the many ways you can approach finding someone to give you advice and guidance. Second, I will...advice on how to “make the ask” once you have found someone you admire and want to learn from. Check out Joanne's...whose career path you are interested in following? Someone you admire for their insight and way of expressing...making the pledge to have lunch or coffee with someone I don’t know very well at least twice a week. I... choose one that comes highly recommended from someone you trust. A good coach can make a big difference...

...give someone a card they are more likely to remember you if you follow up and if you give someone your... to someone and introducing yourself. Put your phone away! This is a natural time to meet someone since... to try and meet. Don’t worry about contacting someone much more senior than you – meeting scientists ...conference may start in the airport. If you see someone traveling with a poster roll, say hello. Keep your...people learn names by seeing them. It is OK to ask someone to see their name tag if you are one of those people...memorable. It isn’t always appropriate to walk up to someone much more senior than you, but you can safely seek...

...doled out to multiple people: someone to coordinate, someone to decide, someone to delegate, etc. If you find... find that there is not someone (or someones) taking on these responsibilities on a team to which you ... subtle but important distinction. If I invite someone to be on a project team, I try to make it very ...kind of team, and you have nagging doubts that someone else on that team is putting their own self-interest...

... your CV or business card. Be warned, though – someone accepting your CV doesn’t necessarily mean that...have a mentor? There is a difference. A mentor is someone who has gone where you hope to go, and who will...made at a conference or job fair, or it may be someone at your institution’s career center. Alternatively...colleague. A mentor does not necessarily need to be someone a lot older than you are; try using ResearchGate... a job! This is good etiquette when contacting someone professionally that you do not know very well; ...

...project. You’ve met someone whose career interests you or you want to pursue someone doing a job you wish...potential to alienate connections. If meeting with someone to ask about a job or a particular position at ...interest in a job. Ask openly to be connected to someone who can help you land that position such as a direct...

...Relationships need nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have a...appreciation. Send a thank you note or gift to someone who helped you succeed in any way. Don’t believe...strawberries for helping with an introduction that helped someone get a job interview. Even just a thank you email...is having a mutual acquaintance connect you to someone who you need to speak to for informational or career...

...relationship. Pro tip: Once you are connected with someone, their email address is usually visible to you ...if you don’t know clearly why you are inviting someone to connect, perhaps you should be asking yourself...discussions.  Pro tip: Instead of connecting with someone you don’t really know, follow instead of connecting...do an easy favor for people in your network. If someone has connected with me, I consider them fair game...

...introduction. During the conference When you meet someone new at a conference, one of the first things you...poster session neighbors. During the session, if someone comes up to my poster, I usually greet them and...scientists attending. Reach out if you’d like to meet someone and set aside a time to meet. Some conferences ...policies before posting something on social media. If someone is presenting unpublished data they may ask the...

...being a manager: yes, being a manager means being someone’s boss. But it does not mean shouting orders and...throughout the year and in a timely manner. If someone’s performance or behavior is inadequate, you need...equally as important so don’t forget to recognize someone’s hard work and reward a job well done. Your report...to your manager. This is when having a mentor - someone more experienced to guide you - is valuable. Protect...

...reports where someone tries to “optimize” a particular standard lab protocol. For example, someone may attempt... the antibody when no signals are obtained. Or someone may heat an ELISA plate during incubation steps...job opportunities (though it is not uncommon for someone to stick with technical support for the long term...

...excitement, in a position. Everyone prefers to hire someone that will be excited to be there. If you are appying..., you will need to be more directed and target someone in a specific department and/or location. The more...asking for help. The worst thing that can happen is someone will say no, but if you don't ask, you will never...

...First, you’ll need to find someone who is willing to host you! If there is someone you personally know or ...scientist? A visiting scientist, in academia, is someone who visits an institution to perform research there...

...Specify desired results. Are you delegating to someone with sufficient skills and experience? Do you think... think you are giving this responsibility to someone who can do the job. It is good to delegate so the...and resources needed for the assignment? Setting someone up for failure by delegating is a bad idea. How...

...research and write your application, then give it to someone else to read and / or give yourself a bit of a ...mentor (your supervisor/ a previous supervisor / someone else in the department) to read over your application...commercial organizations, or universities may have someone senior from accounts or HR on the judging panel...

...for this person’s request? Your colleague is if someone can feed their cells this weekend - why? Do they...plans? What is most important to each person? Someone has to be the first author but another author can...arguing over who gets the spot (a zero sum result - someone doesn’t collect their data), ask why you both want...

...integrity. (Not the case, as I’ve discovered.) As someone who has now seen both sides of the postdoc experience...their pipeline projects. In this scenario, finding someone with whom you can have an in-depth discussion about...companies. Like most jobs, it does help if you know someone who’s already in industry to land you that position...

... similar protocols, and look - they’re seeking someone who has, to a t, all the research skills under ... but also transferable skills, before offering someone their dream job; They want to know if the candidate...happens at your organization, when your boss needs someone to step up, when a new venture presents itself ...